Caspase 7, Apoptosis-Related Cysteine Peptidase (CASP7) antibody
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| Synonyms |
MCH3, CMH-1, ICE-LAP3, Mch3, mCASP-7, AI314680, ICE-IAP3, caspase-7, xCaspase-7, mch3, cmh-1, ice-lap3, CASP7, LOC100101591 |
| Clonality |
Monoclonal (25B204-1) |
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Host
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Reactivity
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Human (127), Mouse (Murine) (66), Rat (Rattus) (63), Pig (Porcine) (39), Cow (Bovine) (37), Dog (Canine) (20), Chicken (18), Horse (Equine) (18), Hamster (3), Monkey (3), Rabbit (2), Sheep (Ovine) (2), Gerbil (1)
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Conjugate
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Biotin (5), FITC (3), HRP (3), Alexa Fluor 350 (2), Alexa Fluor 488 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), Gold (2), PE (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy5.5 (2), PE,Cy7 (2), Alexa Flour 488 (1)
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Application
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 Alternatives Immunoprecipitation (IP), Western Blotting (WB)
Western Blotting (WB) (98), ELISA (40), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (34), Immunofluorescence (IF) (33), Immunohistochemistry (IHC) (24), Immunoprecipitation (IP) (16), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (6), Immunocytochemistry (ICC) (3), Immunoelectron Microscopy (IEM) (2), Flow Cytometry (FACS) (1), Immunohistochemistry (Fixed) (IHC (fx)) (1), Immunohistochemistry (Frozen Sections) (IHC (fro)) (1)
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| Catalog no. |
ABIN121220 |
| Quantity |
0.1 mg (0.5 mg/ml) |
| Price |
330.00 $ Plus shipping costs $45.00
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| Shipping to |
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| Availability |
Will be delivered in 6 to 8 Business Days |
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Alternative name
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Caspase-7
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UniProt
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P55210
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Immunogen
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Recombinant protein of active form of human Caspase-7
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Cross-Reactivity
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Human
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Isotype
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IgG1
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Clone
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25B204-1
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Description
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Caspases are a family of cysteine proteases that are key mediators of programmed cell death or apoptosis. The precursor form of all caspases is composed of a prodomain, and large and small catalytic subunits. The active forms of caspases are generated by several stimuli including ligand-receptor interactions, growth factor deprivation and inhibitors of cellular functions. All known caspases require cleavage adjacent to aspartates to liberate one large and one small subunit, which associate into a2b2 tetramer to form the active enzyme. Caspase-7 is derived from the cleavage of proMCH3-alpha into 2 subunits, p20 and p12 by Caspase-3. Sequence analysis revealed that the MCH3-alpha amino acid sequence had the highest homology to caspase-3 (53% identity) and less than 37% identity with all other family members.
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Application Notes
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Western blot analysis: 1.0 - 2.0 ug/ml. Positive control: Jurkat (a 35 kDa band is observed). Immunoprecipitation: 1 - 2 ug/ml. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
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Concentration
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0.5 mg/ml
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Purification
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Purified
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Buffer
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PBS containing 0.02% sodium azide
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Storage
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Store the antibody at 4°C for one month or at -20°C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.
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Research Area
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Apoptosis/Necrosis
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Restrictions
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For Research Use only
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Fernandes-Alnemri, Takahashi, Armstrong et al.: "Mch3, a novel human apoptotic cysteine protease highly related to CPP32." in: Cancer research, Vol. 55, Issue 24, pp. 6045-52, 1996 (PubMed).
Cohen: "Caspases: the executioners of apoptosis." in: The Biochemical journal, Vol. 326 ( Pt 1), Issue 5693, pp. 1-16, 1997 (PubMed).
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Alternatives for antigen "Caspase 7, Apoptosis-Related Cysteine Peptidase (CASP7)", type "Antibodies"
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Hosts
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Rabbit (109), Mouse (25), Rat (2)
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Reactivities
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Human (127), Mouse (Murine) (66), Rat (Rattus) (63), Pig (Porcine) (39), Cow (Bovine) (37), Dog (Canine) (20), Chicken (18), Horse (Equine) (18), Hamster (3), Monkey (3), Rabbit (2), Sheep (Ovine) (2), Gerbil (1)
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Applications
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Western Blotting (WB) (98), ELISA (40), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (34), Immunofluorescence (IF) (33), Immunohistochemistry (IHC) (24), Immunoprecipitation (IP) (16), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (6), Immunocytochemistry (ICC) (3), Immunoelectron Microscopy (IEM) (2), Flow Cytometry (FACS) (1), Immunohistochemistry (Fixed) (IHC (fx)) (1), Immunohistochemistry (Frozen Sections) (IHC (fro)) (1)
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Conjugates
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Biotin (5), FITC (3), HRP (3), Alexa Fluor 350 (2), Alexa Fluor 488 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), Gold (2), PE (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy5.5 (2), PE,Cy7 (2), Alexa Flour 488 (1)
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Epitopes
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N-Term (16), C-Term (14), Internal Region (4), active (3), Center (2), Cleaved-Asp198 (2), AA 186-198 (1), Cleavage Site (1), Pro-form (1), cleaved (1)
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