Caspase 7, Apoptosis-Related Cysteine Peptidase (CASP7) antibody

Details for Product No. ABIN121220
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(242), (90), (52), (5), (5), (3), (3), (2), (2), (1), (1)
(204), (51), (3)
Clonality (Clone)
Monoclonal ()
(11), (8), (8), (4), (4), (4), (1), (1), (1), (1), (1), (1), (1), (1)
Immunoprecipitation (IP), Western Blotting (WB)
(239), (101), (94), (46), (30), (19), (12), (10), (10), (9), (8), (6), (4), (3), (2), (2)
Pubmed 2 references available
Quantity 0.1 mg
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Catalog No. ABIN121220
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Immunogen Recombinant protein of active form of human Caspase-7
Clone 25B204-1
Isotype IgG1
Purification Protein G Chromatography
Alternative Name Caspase-7
Background Caspases are a family of cysteine proteases that are key mediators of programmed cell death or apoptosis. The precursor form of all caspases is composed of a prodomain, and large and small catalytic subunits. The active forms of caspases are generated by several stimuli including ligand-receptor interactions, growth factor deprivation and inhibitors of cellular functions. All known caspases require cleavage adjacent to aspartates to liberate one large and one small subunit, which associate into a2b2 tetramer to form the active enzyme. Caspase-7 is derived from the cleavage of proMCH3-alpha into 2 subunits, p20 and p12 by Caspase-3. Sequence analysis revealed that the MCH3-alpha amino acid sequence had the highest homology to caspase-3 (53% identity) and less than 37% identity with all other family members.
UniProt P55210
Research Area Apoptosis/Necrosis
Application Notes Western blot analysis: 1.0 - 2.0 ug/ml. Positive control: Jurkat (a 35 kDa band is observed). Immunoprecipitation: 1 - 2 ug/ml. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS containing 0.02% sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody at 4°C for one month or at -20°C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.
Expiry Date 12 months
Supplier Images
anti-Caspase 7, Apoptosis-Related Cysteine Peptidase (CASP7) antibody Western blot analysis for human Caspase-7 using Jurkat cell lysates with ABIN121220 at 2 ug/ml (lane 1) and 0.5 ug/ml (lane 2) dilution. ABIN121220 detects only the 35 kDa caspase-7 corresponding to pro-caspase-7 in non-apoptotic Jurkat cells.
General Cohen: "Caspases: the executioners of apoptosis." in: The Biochemical journal, Vol. 326 ( Pt 1), Issue 5693, pp. 1-16, 1997 (PubMed).

Fernandes-Alnemri, Takahashi, Armstrong et al.: "Mch3, a novel human apoptotic cysteine protease highly related to CPP32." in: Cancer research, Vol. 55, Issue 24, pp. 6045-52, 1996 (PubMed).

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