Caspase 8, Apoptosis-Related Cysteine Peptidase (CASP8) (AA 217-222) antibody

Details for Product No. ABIN121221
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Antigen
Epitope
AA 217-222
(29), (19), (8), (7), (6), (6), (5), (5), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Chimpanzee, Human, Monkey
(218), (73), (58), (25), (13), (12), (12), (9), (5), (2), (1), (1)
Host
Mouse
(186), (54), (6), (2), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(8), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(215), (87), (50), (47), (35), (27), (16), (14), (13), (10), (3), (2), (2), (2), (2), (1), (1)
Pubmed 3 references available
Quantity 0.1 mg
Shipping to United States (Change)
Availability Discontinued
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Catalog No. ABIN121221
500.50 $
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Immunogen Synthetic peptide corresponding to amino acids 217-222 of human caspase-8. AA Sequence:SESQTC
Clone 90A992
Isotype IgG1
Purification Protein G Chromatography
Alternative Name Caspase-8
Background Caspases are a family of cysteine proteases that are key mediators of programmed cell death or apoptosis. The precursor form of all caspases is composed of a prodomain, and large and small catalytic subunits. The active forms of caspases are generated by several stimuli including ligand-receptor interactions, growth factor deprivation and inhibitors of cellular functions. All known caspases require cleavage adjacent to aspartates to liberate one large and one small subunit, which associate into a2b2 tetramer to form the active enzyme. Caspase-8 (FLICE) forms a direct link between the activation of CD95 and the caspase pathway. Overexpression of Caspase-8 induces apoptosis, which can be blocked by inhibitors specific for the ICE family.
UniProt Q14790
Research Area Apoptosis/Necrosis
Application Notes Western blot analysis (0.5 - 2.0 ug/ml, positive control: Jurkat). This antibody can be used for detection of the pro-form of caspase-8. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS containing 0.02% Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.
Expiry Date 12 months
Supplier Images
anti-Caspase 8, Apoptosis-Related Cysteine Peptidase (CASP8) (AA 217-222) antibody Western blot analysis for human Caspase-8 using Jurkat lysates with ABIN121221 at 2 ug/ml (lane 1) and 0.5 ug/ml (lane 2). ABIN121221 only detects 55 kDa caspase-8 in Jurkat cells.
General Muzio, Chinnaiyan, Kischkel et al.: "FLICE, a novel FADD-homologous ICE/CED-3-like protease, is recruited to the CD95 (Fas/APO-1) death--inducing signaling complex." in: Cell, Vol. 85, Issue 6, pp. 817-27, 1996 (PubMed).

Cohen: "Caspases: the executioners of apoptosis." in: The Biochemical journal, Vol. 326 ( Pt 1), Issue 5693, pp. 1-16, 1997 (PubMed).

Li, Nijhawan, Budihardjo et al.: "Cytochrome c and dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade." in: Cell, Vol. 91, Issue 4, pp. 479-89, 1997 (PubMed).

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