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Cytochrome C, Somatic (CYCS) (AA 1-80), (N-Term), (AA 81-104), (AA 66-104) antibody

Details for Product No. ABIN121225
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Antigen
Epitope
AA 1-80, N-Term, AA 81-104, AA 66-104
(8), (6), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Pigeon, Dog (Canine), Horse (Equine), Human, Mammalian, Mouse (Murine), Rabbit, Rat (Rattus)
(114), (72), (67), (32), (20), (16), (13), (12), (12), (7), (5), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Host
Mouse
(109), (63), (7), (4)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(12), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(137), (60), (37), (36), (33), (24), (17), (15), (14), (10), (6), (6), (4), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1)
Pubmed 3 references available
Quantity 0.1 mg
Shipping to United States (Change)
Availability Discontinued
Catalog No. ABIN121225
500.50 $
Plus shipping costs $45.00
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Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen Synthetic peptides corresponding to amino acids 1-80, 81-104 and 66-104 of pigeon CYT were used as the immunogen (Jemmerson et al. 1991)
Clone 7H8-2C12
Isotype IgG2b
Purification Protein G Chromatography.
Alternative Name Cytochrome c
Background In addition to its well-known role in electron transport, Cytochrome C (CYT) has been identified as a mediator of apoptosis or programmed cell death. Cytochrome C translocates from mitochondria into the cytoplasm in the early phases of apoptosis and plays a role in initiation of the apoptotic proteolytic cascade by activating caspase-3. Cytochrome C has been shown to bind Apaf-1. Apaf-1 has also been shown to activate capase-3.
UniProt P99999
Research Area Apoptosis/Necrosis, Metabolism
Application Notes Western blot: 1:500 - 1:1000 (an approx. 15 kDa band is observed). IF/ICC. Recommended Positive Control: HeLa. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS containing 0.05% Sodium Azide as preservative and 0.05% BSA as stabilizer.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.
Expiry Date 12 months
Supplier Images
anti-Cytochrome C, Somatic (CYCS) (AA 1-80), (N-Term), (AA 81-104), (AA 66-104) antibody Western blot detection of Cytochrome C in 15 ugs of HeLa cell lysate using ABIN121225 at 1:1000. A 15 kDa band is detected.
General Fernandes-Alnemri, Litwack, Alnemri: "CPP32, a novel human apoptotic protein with homology to Caenorhabditis elegans cell death protein Ced-3 and mammalian interleukin-1 beta-converting enzyme." in: The Journal of biological chemistry, Vol. 269, Issue 49, pp. 30761-4, 1995 (PubMed).

Liu, Kim, Yang et al.: "Induction of apoptotic program in cell-free extracts: requirement for dATP and cytochrome c." in: Cell, Vol. 86, Issue 1, pp. 147-57, 1996 (PubMed).

Zou, Henzel, Liu et al.: "Apaf-1, a human protein homologous to C. elegans CED-4, participates in cytochrome c-dependent activation of caspase-3." in: Cell, Vol. 90, Issue 3, pp. 405-13, 1997 (PubMed).

Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN121225
500.50 $
Plus shipping costs $45.00
Add to Basket

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Validation Images
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