Cytochrome C, Somatic (CYCS) antibody

Antigen: Cytochrome C, Somatic (CYCS)

Synonyms: cycs, CYCS, CYC, HCS, THC4, cyc, hcs, cyct, MGC75709, MGC89066, DKFZp468F1016, MGC128356, CYCSA, MGC93634, Cyt-c1, CytC-1, CytC1, Cytc, Cytc-d, DC3, bln, bs30f07.y1, cyt c-d, cyt-c-d, cyt.c, cytochrome c, ms(2)04445, pDMc02, DmelCG13263, CG13263, Cyt-c2, CytC-2, Cytc-p, DC4, DMc01, cyt c-p, cyt-c-p, l(2)SH1590, pDMc01, DmelCG17903, CG17903

Clonality: Monoclonal (7H8-2C12)

Host: Mouse

Reactivity: Pigeon, Dog (Canine), Horse (Equine), Human, Mammalian, Mouse (Murine), Rabbit, Rat (Rattus)

Conjugate: Un-conjugated

Application: Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN121225

Additional Information

Alternative name: Cytochrome c

UniProt: P99999

Immunogen: Synthetic peptides corresponding to amino acids 1-80, 81-104 and 66-104 of pigeon CYT were used as the immunogen (Jemmerson et al. 1991)

Cross-Reactivity: Dog (Canine), Horse (Equine), Human, Mammalian, Mouse (Murine), Rabbit, Rat (Rattus)

Isotype: IgG2b

Clone: 7H8-2C12

Description: In addition to its well-known role in electron transport, Cytochrome C (CYT) has been identified as a mediator of apoptosis or programmed cell death. Cytochrome C translocates from mitochondria into the cytoplasm in the early phases of apoptosis and plays a role in initiation of the apoptotic proteolytic cascade by activating caspase-3. Cytochrome C has been shown to bind Apaf-1. Apaf-1 has also been shown to activate capase-3.

Synonyms: cycs, CYCS, CYC, HCS, THC4, cyc, hcs, cyct, MGC75709, MGC89066, DKFZp468F1016, MGC128356, CYCSA, MGC93634, Cyt-c1, CytC-1, CytC1, Cytc, Cytc-d, DC3, bln, bs30f07.y1, cyt c-d, cyt-c-d, cyt.c, cytochrome c, ms(2)04445, pDMc02, DmelCG13263, CG13263, Cyt-c2, CytC-2, Cytc-p, DC4, DMc01, cyt c-p, cyt-c-p, l(2)SH1590, pDMc01, DmelCG17903, CG17903

Application Details

Application Notes: Western blot: 1:500 - 1:1000 (an approx. 15 kDa band is observed). IF/ICC. Recommended Positive Control: HeLa. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.5 mg/ml

Purification: Purified

Buffer: PBS, 0.05% sodium azide

Storage: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.

Research Area: Apoptosis/Necrosis, Metabolism

Restrictions: For Research Use only

Images

Western blot detection of Cytochrome C in 15 ugs of HeLa cell lysate using ABIN121225 at 1:1000. A 15 kDa band is detected.

Publications

Fernandes-Alnemri, Litwack, Alnemri: "CPP32, a novel human apoptotic protein with homology to Caenorhabditis elegans cell death protein Ced-3 and mammalian interleukin-1 beta-converting enzyme." in: The Journal of biological chemistry, Vol. 269, Issue 49, pp. 30761-4, 1995 (PubMed).

Liu, Kim, Yang et al.: "Induction of apoptotic program in cell-free extracts: requirement for dATP and cytochrome c." in: Cell, Vol. 86, Issue 1, pp. 147-57, 1996 (PubMed).

Zou, Henzel, Liu et al.: "Apaf-1, a human protein homologous to C. elegans CED-4, participates in cytochrome c-dependent activation of caspase-3." in: Cell, Vol. 90, Issue 3, pp. 405-13, 1997 (PubMed).