Death-Associated Protein 3 (DAP3) antibody

Antigen: Death-Associated Protein 3 (DAP3)

Synonyms: DAP6, EAP1, BING2, MGC126245, MGC126246, DAP-3, 4921514D13Rik, MRPS29, MRP-S29, bMRP-10, MGC126058, MGC126059, DKFZp686G12159, DAP3

Clonality: Monoclonal (42C617)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Catalog no.: ABIN121228

Additional Information

Alternative name: DAP3 / MRPS29

UniProt: P51398

Immunogen: Synthetic peptide corresponding to amino acids 51-66 of human DAP-3 protein

Cross-Reactivity: Human, Mouse (Murine)

Isotype: IgG

Clone: 42C617

Description: Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Recently, five novel apoptotic genes (named DAP genes for: Death Associated Proteins) have been identified that code for proteins, which display a diverse spectrum of biochemical activities. These include a novel type of calcium/calmodulin-regulated kinase which carries ankyrin repeats and a death domain (DAP- kinase), a nucleotide-binding protein (DAP-3), a small proline-rich cytoplasmic protein (DAP-1), and a novel homolog of the eIF4G translation initiation factor (DAP-5) (1). DAP-3 has an ATP/GTP binding motive (P-loop) and has been isolated from human and mouse, and named as hDAP-3 and mDAP-3 (Death Associated Protein-3), respectively (2-3). Sequence analysis revealed an 82% amino acid identity between human and mouse protein. In situ hybridization and northern blot analysis showed an abundant mRNA expression with a pronounced expression in highly proliferative epithelial compartments. Overexpression of DAP-3 leads to cytochrome c release and induction of cell death. DAP-3 mediated apoptosis was shown to depend on a functional P- loop. mDAP-3 is localized to the mitochondrial matrix and in contrast to cytochrome c, retained its mitochondrial localization during apoptosis induction (3).

Specificity: This antibody is specific for DAP-3. This sequence is 75% homologous in mouse and rat

Application Details

Application Notes: Western blot analysis: 1.0 - 2.0 ug/ml. Positive control: Jurkat (a 45 kDa band is observed). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.5 mg/ml

Purification: Purified

Buffer: PBS containing 0.02 % sodium azide

Storage: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.

Restrictions: For Research Use only

Images

Western blot analysis of 10 ug of total cell lysate from Jurkat cells with ABIN121228 at 1 ug/ml.

Publications

Kissil, Deiss, Bayewitch et al.: "Isolation of DAP3, a novel mediator of interferon-gamma-induced cell death." in: The Journal of biological chemistry, Vol. 270, Issue 46, pp. 27932-6, 1996 (PubMed).

Kimchi: "DAP kinase and DAP-3: novel positive mediators of apoptosis." in: Annals of the rheumatic diseases, Vol. 58 Suppl 1, pp. I14-9, 2000 (PubMed).

Berger, Brigl, Herrmann et al.: "The apoptosis mediator mDAP-3 is a novel member of a conserved family of mitochondrial proteins." in: Journal of cell science, Vol. 113 ( Pt 20), pp. 3603-12, 2000 (PubMed).