Eukaryotic Translation Initiation Factor 4 gamma 2 (EIF4G2) (AA 796-812) antibody

Details for Product No. ABIN121229
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Antigen
Epitope
AA 796-812
(7), (6), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine)
(66), (30), (21), (3), (3), (2), (2), (1)
Host
Mouse
(50), (16)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(55), (23), (18), (14), (10), (8), (3), (2), (1), (1)
Pubmed 4 references available
Quantity 0.1 mg
Shipping to United States (Change)
Availability Discontinued
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Catalog No. ABIN121229
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Immunogen Two synthetic peptides corresponding to amino acids 796-812 of human DAP-5 protein
Clone 39C534
Isotype IgG1
Purification Protein G Chromatography
Alternative Name eIF4G2 / DAP5
Background Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Recently, five novel apoptotic genes (named DAP genes for: Death Associated Proteins) have been identified that code for proteins, which display a diverse spectrum of biochemical activities. These include a novel type of calcium/calmodulin-regulated kinase, which carries ankyrin repeats and a death domain (DAP- kinase), a nucleotide-binding protein (DAP-3), a small proline-rich cytoplasmic protein (DAP-1), and a novel homolog of the eIF4G translation initiation factor (DAP-5) (1-2). DAP-5 (also named p97 and NAT1) is a ubiquitously expressed member of the translation initiation factor eIF4G family that lacks the eIF4E binding site. DAP-5 was cloned from HeLa cells using a functional approach in attempt to isolate cDNA fragments, which convey resistance to gamma interferon (IFN-g)-induced programmed cell death (3). A fragment of the cDNA when expressed as a mini- protein of 28 kDa protects the cell from IFN-g induced apoptosis. The full-length DAP-5 cDNA codes for a protein of approximate molecular weight of 97 kDa. Low level of expression protects the cells, whereas high level of expression results in cell death (3-4).
UniProt P78344
Application Notes Western blot analysis: 1.0 - 2.0 ug/ml. Positive control: Jurkat (a 97 kDa band is observed). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS containing 0.2 % gelatin, 0.05 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.
Expiry Date 12 months
Supplier Images
anti-Eukaryotic Translation Initiation Factor 4 gamma 2 (EIF4G2) (AA 796-812) antibody Western blot analysis of 15 ug of total cell lysate from Jurkat cells with ABIN121229 at 1 ug/ml.
General Levy-Strumpf, Kimchi: "Death associated proteins (DAPs): from gene identification to the analysis of their apoptotic and tumor suppressive functions." in: Oncogene, Vol. 17, Issue 25, pp. 3331-40, 1999 (PubMed).

Kimchi: "DAP kinase and DAP-3: novel positive mediators of apoptosis." in: Annals of the rheumatic diseases, Vol. 58 Suppl 1, pp. I14-9, 2000 (PubMed).

Berger, Brigl, Herrmann et al.: "The apoptosis mediator mDAP-3 is a novel member of a conserved family of mitochondrial proteins." in: Journal of cell science, Vol. 113 ( Pt 20), pp. 3603-12, 2000 (PubMed).

Wittke, Maedge, Wiedemeyer et al.: "DAP-5 is involved in MycN/IFNgamma-induced apoptosis in human neuroblastoma cells." in: Cancer letters, Vol. 162, Issue 2, pp. 237-43, 2001 (PubMed).

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