Eukaryotic Translation Initiation Factor 4 gamma 2 (EIF4G2) antibody

Antigen: Eukaryotic Translation Initiation Factor 4 gamma 2 (EIF4G2)

Synonyms: p97, AAG1, DAP5, NAT1, FLJ41344, EIF4G2, NAT1B, eif4g2, hm:zeh1307, wu:fa14h01, wu:fb44h01, wu:fb59c06, wu:fc21b05, DKFZp459D102

Clonality: Monoclonal (39C534)

Host: Mouse

Reactivity: Human, Mouse (Murine)

Application: Western Blotting (WB)

Catalog no.: ABIN121229

Additional Information

Alternative name: EIF4G2 / DAP5

Swiss-Prot: P78344

Immunogen: Two synthetic peptides corresponding to amino acids 796-812 of human DAP-5 protein

Isotype: IgG1  (Matching secondary antibodies)

Clone: 39C534

Description: Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Recently, five novel apoptotic genes (named DAP genes for: Death Associated Proteins) have been identified that code for proteins, which display a diverse spectrum of biochemical activities. These include a novel type of calcium/calmodulin-regulated kinase, which carries ankyrin repeats and a death domain (DAP- kinase), a nucleotide-binding protein (DAP-3), a small proline-rich cytoplasmic protein (DAP-1), and a novel homolog of the eIF4G translation initiation factor (DAP-5) (1-2). DAP-5 (also named p97 and NAT1) is a ubiquitously expressed member of the translation initiation factor eIF4G family that lacks the eIF4E binding site. DAP-5 was cloned from HeLa cells using a functional approach in attempt to isolate cDNA fragments, which convey resistance to gamma interferon (IFN-g)-induced programmed cell death (3). A fragment of the cDNA when expressed as a mini- protein of 28 kDa protects the cell from IFN-g induced apoptosis. The full-length DAP-5 cDNA codes for a protein of approximate molecular weight of 97 kDa. Low level of expression protects the cells, whereas high level of expression results in cell death (3-4).

Specificity: This antibody is specific for DAP-5.

Application Details

Application Notes: Western blot analysis: 1.0 - 2.0 ug/ml. Positive control: Jurkat (a 97 kDa band is observed). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.5 mg/ml

Buffer: PBS containing 0.2 % gelatin, 0.05 % sodium azide

Storage: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.

Restrictions: For Research Use only

Images

Western blot analysis of 15 ug of total cell lysate from Jurkat cells with ABIN121229 at 1 ug/ml.

Publications

Publications: Levy-Strumpf, Kimchi: "Death associated proteins (DAPs): from gene identification to the analysis of their apoptotic and tumor suppressive functions." in: Oncogene, Vol. 17, Issue 25, pp. 3331-40, 1999 (PubMed).

Kimchi: "DAP kinase and DAP-3: novel positive mediators of apoptosis." in: Annals of the rheumatic diseases, Vol. 58 Suppl 1, pp. I14-9, 2000 (PubMed).

Berger, Brigl, Herrmann et al.: "The apoptosis mediator mDAP-3 is a novel member of a conserved family of mitochondrial proteins." in: Journal of cell science, Vol. 113 ( Pt 20), pp. 3603-12, 2000 (PubMed).

Wittke, Maedge, Wiedemeyer et al.: "DAP-5 is involved in MycN/IFNgamma-induced apoptosis in human neuroblastoma cells." in: Cancer letters, Vol. 162, Issue 2, pp. 237-43, 2001 (PubMed).