Tumor Necrosis Factor Receptor Superfamily, Member 6b, Decoy (TNFRSF6B) antibody

Antigen: Tumor Necrosis Factor Receptor Superfamily, Member 6b, Decoy (TNFRSF6B)

Synonyms: M68, TR6, DCR3, DJ583P15.1.1, TNFRSF6B

Clonality: Monoclonal (37A565)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Catalog no.: ABIN121230

Additional Information

Alternative name: TNFRSF6B / DCR3

UniProt: O95407

Immunogen: A full-length recombinant protein of human DcR3

Cross-Reactivity: Human

Isotype: IgG

Clone: 37A565

Description: Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Apoptosis is induced by certain cytokines including TNF and Fas ligand (FasL) of the TNF family through their death domain containing receptors, TNFR1 and Fas (also known as CD95 or Apo-1). Recently, Pitti et al., 1998 have identified a molecule that can bind to FasL and block its binding to Fas (1). This molecule called, decoy receptor 3 (DcR3), is generally amplified in a number of lung and colon carcinomas suggesting that certain tumors may escape FasL-dependent cell death by expressing a decoy receptor that blocks FasL (1,2). An identical molecule was isolated by Yu et al, 1999 that also binds to FasL and another cellular ligand, LIGHT (herpes virus entry mediator (HVEM)-L) (3,4). It has been shown that LIGHT induces apoptosis of various tumor cells that express both lymphotoxinb receptor and HVEM/TR2 receptors. DcR3/TR6 is constitutively expressed in lung tissue, tumor cells and in endothelial cells (1,3). Like other members of TNFR superfamily, DcR1, DcR2 and OPG, DcR3/TR6 may act as an inhibitor of signaling through TNF family members, FasL and LIGHT. However, unlike DcR1 and DcR2, which are membrane-associated proteins, DcR3 is directly secreted into the extracellular space.

Application Details

Application Notes: Western blot analysis: 2.0 - 4.0 ug/ml. Positive control: Jurkat (a 34 kDa band is observed). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.5 mg/ml

Purification: Purified

Buffer: PBS containing 0.02% sodium azide

Storage: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.

Restrictions: For Research Use only

Images

Western blot analysis of 30 ug of total cell lysate from Jurkat cells with ABIN121230 at 2 ug/ml.

Publications

Mauri, Ebner, Montgomery et al.: "LIGHT, a new member of the TNF superfamily, and lymphotoxin alpha are ligands for herpesvirus entry mediator." in: Immunity, Vol. 8, Issue 1, pp. 21-30, 1998 (PubMed).

Green: "Apoptosis. Death deceiver." in: Nature, Vol. 396, Issue 6712, pp. 629-30, 1999 (PubMed).

Yu, Kwon, Ni et al.: "A newly identified member of tumor necrosis factor receptor superfamily (TR6) suppresses LIGHT-mediated apoptosis." in: The Journal of biological chemistry, Vol. 274, Issue 20, pp. 13733-6, 1999 (PubMed).