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Details for Product No. ABIN121231

DNA Fragmentation Factor, 40kDa, beta Polypeptide (Caspase-Activated DNase) (DFFB) (AA 205-222) antibody

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Antigen
Synonyms CAD, CPAN, DFF2, DFF40, DFF-40, 40kDa, Didff, 5730477D02Rik, Cad, LOC100223514
Epitope
»Alternatives AA 205-222
Reactivity
»Alternatives Human
Host
»Alternatives Mouse
Clonality (Clone) Monoclonal ()
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives Western Blotting (WB)
Pubmed 5 references available
Catalog no. ABIN121231
Quantity 0.1 mg
Price
445.50 $   Plus shipping costs $45.00
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Availability Will be delivered in 6 to 8 Business Days
Immunogen Peptide corresponding to amino acids 205-222 of human DFF40
Clone 36A349
Isotype IgG1
Purification Protein G Chromatography
Alternative Name DFFB / CAD
Background Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A mouse DNase that causes DNA fragmentation was identified recently and designated CAD (for caspase activated deoxyribo-nuclease) (1,2). The human homologue of mouse CAD was more recently identified by two groups independently and termed CPAN and DFF40 (3,4). Human DFF45 and its mouse homologue ICAD are the inhibitors of CPAN/DFF40 and CAD, respectively (5). Upon cleavage of DFF45/ICAD by activated caspases, DFF40/CAD is released and activated and eventually causes degradation of DNA in the nuclei1 (4). Activation of CAD/DFF40, which causes DNA degradation, is the hallmark of apoptotic cell death.
UniProt O76075
Application Notes Western blot (2 ug/ml, positive control: HeLa cells). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS containing 0.02% sodium azide
Preservative Sodium azide
Storage 4 °C/-20 °C
Storage Comment Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.
Expiry Date 12 months
Liu, Zou, Slaughter et al.: "DFF, a heterodimeric protein that functions downstream of caspase-3 to trigger DNA fragmentation during apoptosis." in: Cell, Vol. 89, Issue 2, pp. 175-84, 1997 (PubMed).

Enari, Sakahira, Yokoyama et al.: "A caspase-activated DNase that degrades DNA during apoptosis, and its inhibitor ICAD." in: Nature, Vol. 391, Issue 6662, pp. 43-50, 1998 (PubMed).

Sakahira, Enari, Nagata: "Cleavage of CAD inhibitor in CAD activation and DNA degradation during apoptosis." in: Nature, Vol. 391, Issue 6662, pp. 96-9, 1998 (PubMed).

Halenbeck, MacDonald, Roulston et al.: "CPAN, a human nuclease regulated by the caspase-sensitive inhibitor DFF45." in: Current biology : CB, Vol. 8, Issue 9, pp. 537-40, 1998 (PubMed).

Liu, Li, Widlak et al.: "The 40-kDa subunit of DNA fragmentation factor induces DNA fragmentation and chromatin condensation during apoptosis." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 15, pp. 8461-6, 1998 (PubMed).

Alternatives for antigen "DNA Fragmentation Factor, 40kDa, beta Polypeptide (Caspase-Activated DNase) (DFFB)", type "Antibodies"
Hosts (108), (12)
Reactivities (79), (71), (44), (13), (11), (1), (1)
Applications (99), (33), (28), (20), (19), (11), (8), (4), (2), (1), (1), (1), (1)
Conjugates (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Epitopes (11), (11), (9), (7), (6), (4), (4), (4), (3), (2), (1), (1), (1), (1)
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