Add to Basket
|+1 404 474 4654|
|+1 888 205 9894 (TF)|
DNA Fragmentation Factor, 40kDa, beta Polypeptide (Caspase-Activated DNase) (DFFB) antibody
|Synonyms||CAD, CPAN, DFF2, DFF40, DFF-40, DFFB, LOC100223514|
Alternatives Western Blotting (WB)
|5 references available|
|Quantity||0.1 mg (0.5 mg/ml)|
|Price||319.00 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 6 to 8 Business Days|
|Alternative name||DFFB / CAD|
|Immunogen||Peptide corresponding to amino acids 205-222 of human DFF40|
|Description||Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A mouse DNase that causes DNA fragmentation was identified recently and designated CAD (for caspase activated deoxyribo-nuclease) (1,2). The human homologue of mouse CAD was more recently identified by two groups independently and termed CPAN and DFF40 (3,4). Human DFF45 and its mouse homologue ICAD are the inhibitors of CPAN/DFF40 and CAD, respectively (5). Upon cleavage of DFF45/ICAD by activated caspases, DFF40/CAD is released and activated and eventually causes degradation of DNA in the nuclei1 (4). Activation of CAD/DFF40, which causes DNA degradation, is the hallmark of apoptotic cell death.|
|Specificity||This antibody is specific for DFF-40/CAD/CPAN.|
|Application Notes||Western blot (2 ug/ml, positive control: HeLa cells). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.|
|Buffer||PBS containing 0.02% sodium azide|
|Storage||Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.|
|Restrictions||For Research Use only|
|Western blot analysis of 30 ug of HeLa cell lysate with antibody ABIN121231 at a dilution of 2 ug/ml. A 40 kDa band is observed.|
Liu, Zou, Slaughter et al.: "DFF, a heterodimeric protein that functions downstream of caspase-3 to trigger DNA fragmentation during apoptosis." in: Cell, Vol. 89, Issue 2, pp. 175-84, 1997 (PubMed).
Enari, Sakahira, Yokoyama et al.: "A caspase-activated DNase that degrades DNA during apoptosis, and its inhibitor ICAD." in: Nature, Vol. 391, Issue 6662, pp. 43-50, 1998 (PubMed).
Sakahira, Enari, Nagata: "Cleavage of CAD inhibitor in CAD activation and DNA degradation during apoptosis." in: Nature, Vol. 391, Issue 6662, pp. 96-9, 1998 (PubMed).
Halenbeck, MacDonald, Roulston et al.: "CPAN, a human nuclease regulated by the caspase-sensitive inhibitor DFF45." in: Current biology : CB, Vol. 8, Issue 9, pp. 537-40, 1998 (PubMed).
Liu, Li, Widlak et al.: "The 40-kDa subunit of DNA fragmentation factor induces DNA fragmentation and chromatin condensation during apoptosis." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 15, pp. 8461-6, 1998 (PubMed).
|Hosts||Rabbit (58), Mouse (6)|
|Reactivities||Human (45), Mouse (Murine) (41), Rat (Rattus) (24), Guinea Pig (1)|
|Applications||Western Blotting (WB) (47), Immunofluorescence (IF) (19), ELISA (13), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (9), Immunohistochemistry (IHC) (7), Immunocytochemistry (ICC) (6), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Dot Blot (Dot) (1), Immunoelectron Microscopy (IEM) (1), Immunoprecipitation (IP) (1)|
|Conjugates||Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)|
|Epitopes||AA 205-222 (9), AA 314-329 (4), Internal Region (4), N-Term (4), AA 147-164 (2), AA 203-218 (2), AA 3-18 (2), C-Term (2), Middle Region (2)|