Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA) antibody

Antigen: Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA)

Synonyms: IKBA, MAD-3, NFKBI

Clonality: Monoclonal (6A920)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Catalog no.: ABIN121247

Additional Information

Alternative name: NFKBIA / IKBA

UniProt: P25963

Immunogen: Recombinant protein corresponding to amino acid residues 32-291 of human IkBa

Cross-Reactivity: Human, Mouse (Murine)

Isotype: IgG1

Clone: 6A920

Description: NF-kB is silenced in the cytoplasm by an inhibitory protein, IkB (1). Synthesis of IkBa is autoregulated (2). IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g (3-6). External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Six members of IkB family members have been identified (1). One of the first gene induced following NF-kB activation is IkBa.

Specificity: The antibody reacts with IkBa.

Application Details

Application Notes: Immunoprecipitation: 1 ug/ml. Western blot (1.0 - 2.0 ug/ml). Positive control: Daudi, Jurkat, or NIH 3T3 (40 kDa band is observed). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.5 mg/ml

Purification: Purified

Buffer: PBS containing 0.05 % BSA, 0.05 % sodium azide

Storage: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.

Restrictions: For Research Use only

Images

Western blot analysis of IkBa using ABIN121247 at 1 ug/mL against 30 ug of Jurkat total cell lysate.

Publications

DiDonato, Hayakawa, Rothwarf et al.: "A cytokine-responsive IkappaB kinase that activates the transcription factor NF-kappaB." in: Nature, Vol. 388, Issue 6642, pp. 548-54, 1997 (PubMed).

Verma, Stevenson: "IkappaB kinase: beginning, not the end." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 22, pp. 11758-60, 1997 (PubMed).

Mercurio, Zhu, Murray et al.: "IKK-1 and IKK-2: cytokine-activated IkappaB kinases essential for NF-kappaB activation." in: Science (New York, N.Y.), Vol. 278, Issue 5339, pp. 860-6, 1997 (PubMed).

Yamaoka, Courtois, Bessia et al.: "Complementation cloning of NEMO, a component of the IkappaB kinase complex essential for NF-kappaB activation." in: Cell, Vol. 93, Issue 7, pp. 1231-40, 1998 (PubMed).

Sanjo, Kawai, Akira: "DRAKs, novel serine/threonine kinases related to death-associated protein kinase that trigger apoptosis." in: The Journal of biological chemistry, Vol. 273, Issue 44, pp. 29066-71, 1998 (PubMed).

Moriguchi, Urushiyama, Hisamoto et al.: "WNK1 regulates phosphorylation of cation-chloride-coupled cotransporters via the STE20-related kinases, SPAK and OSR1." in: The Journal of biological chemistry, Vol. 280, Issue 52, pp. 42685-93, 2005 (PubMed).