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IKBKB antibody (Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase beta)

Details for Product anti-IKBKB Antibody No. ABIN121248, Supplier: Login to see New
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(608), (152), (122), (14), (8), (2), (2), (1), (1)
(550), (72), (9), (2)
Clonality (Clone)
Monoclonal ()
This IKBKB antibody is un-conjugated
(36), (36), (36), (31), (31), (31), (9), (5), (5), (5), (5), (5), (5), (5), (5)
Immunoprecipitation (IP), Western Blotting (WB), Flow Cytometry (FACS)
(297), (265), (238), (83), (62), (46), (45), (24), (15), (6), (5), (4), (3), (3), (1), (1)
Pubmed 3 references available
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Quantity 0.1 mg
Shipping to United States ( )
Immunogen Full-length human IKKb recombinant protein
Clone 10AG2
Isotype IgG1
Purification Protein G chromatography
Alternative Name IKBKB / IKKB (IKBKB Antibody Abstract)
Background NF-kB (nuclear factor kB) is sequestered in the cytoplasm by IkB family of inhibitory proteins that mask the nuclear localization signal of NF-kB thereby preventing translocation of NF-kB to the nucleus (1). External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Synthesis of IkBa is autoregulated (2). IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g (3-5). IKK1/a and IKK2/b are phosphorylated by NF-kB-inducing kinase (NIK) (6) and MAP kinase kinase kinase-1 (MEKK1) (7), respectively. Recent studies have shown that IKK2/b, not IKK1/a, is the target proinflammatory stimuli (9). Targeted disruption of IKK2/b gene in mice also results in extensive liver damage from apotopsis and death as embryo (9).
UniProt O14920
Pathways NF-kappaB Signaling, RTK Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway
Application Notes Western blot (2 - 4 ug/ml). Immunoprecipitation. Flow (intracellular). Recommended Positive Control : Daudi, HeLa, NIH 3T3 (a ~87 kDa band should be observed). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS containing 0.05 % BSA, 0.05 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.
Expiry Date 12 months
Supplier Images
Western Blotting (WB) image for anti-IKBKB antibody (Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase beta) (ABIN121248) Western blot analysis of 30 µg of total cell lysate from A) Daudi, B) HeLa, and C) mo...
General Yu, Kwon, Ni et al.: "A newly identified member of tumor necrosis factor receptor superfamily (TR6) suppresses LIGHT-mediated apoptosis." in: The Journal of biological chemistry, Vol. 274, Issue 20, pp. 13733-6, 1999 (PubMed).

Green: "Apoptosis. Death deceiver." in: Nature, Vol. 396, Issue 6712, pp. 629-30, 1999 (PubMed).

Mauri, Ebner, Montgomery et al.: "LIGHT, a new member of the TNF superfamily, and lymphotoxin alpha are ligands for herpesvirus entry mediator." in: Immunity, Vol. 8, Issue 1, pp. 21-30, 1998 (PubMed).

Catalog No. ABIN121248

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