Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase beta (IKBKB) antibody

Antigen: Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase beta (IKBKB)

Synonyms: IKK2, IKKB, NFKBIKB, FLJ40509, IKK-beta, MGC131801, ikk2, ikkb, ikkbeta, MGC80376, ikk-beta, IKBKB, DIK, DLAK, DmIKK, DmIKKBeta, DmIKKb, IK, IKK, IKKbeta, Ird-5, Ird5, LRK, Lak, anon-89Bd, dIKK, dIKK-beta, ird, ird5/DmIkk[beta], DmelCG4201, CG4201

Clonality: Monoclonal (10AG2)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Immunoprecipitation (IP), Western Blotting (WB), Flow Cytometry (FACS)

Catalog no.: ABIN121248

Additional Information

Alternative name: IKBKB / IKKB

UniProt: O14920

Immunogen: Full-length human IKKb recombinant protein

Cross-Reactivity: Human

Isotype: IgG1

Clone: 10AG2

Description: NF-kB (nuclear factor kB) is sequestered in the cytoplasm by IkB family of inhibitory proteins that mask the nuclear localization signal of NF-kB thereby preventing translocation of NF-kB to the nucleus (1). External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Synthesis of IkBa is autoregulated (2). IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g (3-5). IKK1/a and IKK2/b are phosphorylated by NF-kB-inducing kinase (NIK) (6) and MAP kinase kinase kinase-1 (MEKK1) (7), respectively. Recent studies have shown that IKK2/b, not IKK1/a, is the target proinflammatory stimuli (9). Targeted disruption of IKK2/b gene in mice also results in extensive liver damage from apotopsis and death as embryo (9).

Synonyms: IKK2, IKKB, NFKBIKB, FLJ40509, IKK-beta, MGC131801, ikk2, ikkb, ikkbeta, MGC80376, ikk-beta, IKBKB, DIK, DLAK, DmIKK, DmIKKBeta, DmIKKb, IK, IKK, IKKbeta, Ird-5, Ird5, LRK, Lak, anon-89Bd, dIKK, dIKK-beta, ird, ird5/DmIkk[beta], DmelCG4201, CG4201

Application Details

Application Notes: Western blot (2 - 4 ug/ml). Immunoprecipitation. Flow (intracellular). Recommended Positive Control : Daudi, HeLa, NIH 3T3 (a ~87 kDa band should be observed). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.5 mg/ml

Purification: Purified

Buffer: PBS containing 0.05 % BSA, 0.05 % sodium azide

Storage: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.

Restrictions: For Research Use only

Images

Western blot analysis of 30 µg of total cell lysate from A) Daudi, B) HeLa, and C) mouse NIH 3T3 cells using ABIN121248 at 2 ug/ml dilution.

Publications

Mauri, Ebner, Montgomery et al.: "LIGHT, a new member of the TNF superfamily, and lymphotoxin alpha are ligands for herpesvirus entry mediator." in: Immunity, Vol. 8, Issue 1, pp. 21-30, 1998 (PubMed).

Green: "Apoptosis. Death deceiver." in: Nature, Vol. 396, Issue 6712, pp. 629-30, 1999 (PubMed).

Yu, Kwon, Ni et al.: "A newly identified member of tumor necrosis factor receptor superfamily (TR6) suppresses LIGHT-mediated apoptosis." in: The Journal of biological chemistry, Vol. 274, Issue 20, pp. 13733-6, 1999 (PubMed).