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|+1 404 474 4654|
|+1 888 205 9894 (TF)|
Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase beta (IKBKB) antibody
Human, Mouse (Murine)
Alternatives Immunoprecipitation (IP), Western Blotting (WB), Flow Cytometry (FACS)
|9 references available|
|Quantity||0.1 mg (0.5 mg/ml)|
|Price||341.00 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 6 to 8 Business Days|
|Alternative name||IKBKB / IKKB|
|Immunogen||Full-length human IKKb/IKK2 recombinant protein|
|Cross-Reactivity||Human, Mouse (Murine)|
|Description||NF-kB (nuclear factor kB) is sequestered in the cytoplasm by IkB family of inhibitory proteins that mask the nuclear localization signal of NF-kB thereby preventing translocation of NF-kB to the nucleus (1). External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Synthesis of IkBa is autoregulated (2). IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g (3-5). IKK1/a and IKK2/b are phosphorylated by NF-kB-inducing kinase (NIK) (6) and MAP kinase kinase kinase-1 (MEKK1) (7), respectively. Recent studies have shown that IKK2/b, not IKK1/a, is the target proinflammatory stimuli (9). Targeted disruption of IKK2/b gene in mice also results in extensive liver damage from apotopsis and death as embryo (9).|
|Synonyms||IKK2, IKKB, NFKBIKB, FLJ40509, IKK-beta, MGC131801, ikk2, ikkb, ikkbeta, MGC80376, ikk-beta, IKBKB, DIK, DLAK, DmIKK, DmIKKBeta, DmIKKb, IK, IKK, IKKbeta, Ird-5, Ird5, LRK, Lak, anon-89Bd, dIKK, dIKK-beta, ird, ird5/DmIkk[beta], DmelCG4201, CG4201|
|Application Notes||Western blot (2 - 5 ug/ml). Immunoprecipitation ( 2-5 ug/1x10e6 cells). Recommended Positive Control: Daudi, HeLa, Jurkat, or NIH 3T3 (an ~87 kDa band should be observed). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.|
|Buffer||PBS containing 0.2% gelatin, 0.05% sodium azide|
|Storage||Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.|
|Restrictions||For Research Use only|
|ABIN121249 was used to immunoprecipitate IKKb from 1x10e6 Daudi cells and the immuno-precipitated protein was detected by western blotting using ABIN121248. IKKb was detected as a protein of ~87 kD.|
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