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IKBKB antibody (Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase beta)

Details for Product anti-IKBKB Antibody No. ABIN121249, Supplier: Login to see New
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Human, Mouse (Murine)
(608), (152), (122), (14), (8), (2), (2), (1), (1)
(550), (72), (9), (2)
Clonality (Clone)
Monoclonal ()
This IKBKB antibody is un-conjugated
(36), (36), (36), (31), (31), (31), (9), (5), (5), (5), (5), (5), (5), (5), (5)
Immunoprecipitation (IP), Western Blotting (WB), Flow Cytometry (FACS)
(297), (265), (238), (83), (62), (46), (45), (24), (15), (6), (5), (4), (3), (3), (1), (1)
Pubmed 9 references available
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Catalog number from supplier Login to see New
Quantity 0.1 mg
Shipping to United States ( )
Immunogen Full-length human IKKb/IKK2 recombinant protein
Clone 10A9B6
Isotype IgG1
Purification Protein G Chromatography
Alternative Name IKBKB / IKKB (IKBKB Antibody Abstract)
Background NF-kB (nuclear factor kB) is sequestered in the cytoplasm by IkB family of inhibitory proteins that mask the nuclear localization signal of NF-kB thereby preventing translocation of NF-kB to the nucleus (1). External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Synthesis of IkBa is autoregulated (2). IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g (3-5). IKK1/a and IKK2/b are phosphorylated by NF-kB-inducing kinase (NIK) (6) and MAP kinase kinase kinase-1 (MEKK1) (7), respectively. Recent studies have shown that IKK2/b, not IKK1/a, is the target proinflammatory stimuli (9). Targeted disruption of IKK2/b gene in mice also results in extensive liver damage from apotopsis and death as embryo (9).
UniProt O14920
Pathways NF-kappaB Signaling, RTK Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway
Application Notes Western blot (2 - 5 ug/ml). Immunoprecipitation ( 2-5 ug/1x10e6 cells). Recommended Positive Control: Daudi, HeLa, Jurkat, or NIH 3T3 (an ~87 kDa band should be observed). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS containing 0.05% BSA and 0.05% Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.
Expiry Date 12 months
Supplier Images
Western Blotting (WB) image for anti-IKBKB antibody (Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase beta) (ABIN121249) ABIN121249 was used to immunoprecipitate IKKb from 1x10e6 Daudi cells and the immuno-...
General Muta, Kang, Kitajima et al.: "p32 protein, a splicing factor 2-associated protein, is localized in mitochondrial matrix and is functionally important in maintaining oxidative phosphorylation." in: The Journal of biological chemistry, Vol. 272, Issue 39, pp. 24363-70, 1997 (PubMed).

Georgopoulos, Hendrix, Casjens et al.: "Host participation in bacteriophage lambda head assembly." in: Journal of molecular biology, Vol. 76, Issue 1, pp. 45-60, 1973 (PubMed).

McMullin, Hallberg: "A normal mitochondrial protein is selectively synthesized and accumulated during heat shock in Tetrahymena thermophila." in: Molecular and cellular biology, Vol. 7, Issue 12, pp. 4414-23, 1988 (PubMed).

Hemmingsen, Woolford, van der Vies et al.: "Homologous plant and bacterial proteins chaperone oligomeric protein assembly." in: Nature, Vol. 333, Issue 6171, pp. 330-4, 1988 (PubMed).

Cheng, Hartl, Martin et al.: "Mitochondrial heat-shock protein hsp60 is essential for assembly of proteins imported into yeast mitochondria." in: Nature, Vol. 337, Issue 6208, pp. 620-5, 1989 (PubMed).

Mizzen, Chang, Garrels et al.: "Identification, characterization, and purification of two mammalian stress proteins present in mitochondria, grp 75, a member of the hsp 70 family and hsp 58, a homolog of the bacterial groEL protein." in: The Journal of biological chemistry, Vol. 264, Issue 34, pp. 20664-75, 1990 (PubMed).

Manning-Krieg, Scherer, Schatz: "Sequential action of mitochondrial chaperones in protein import into the matrix." in: The EMBO journal, Vol. 10, Issue 11, pp. 3273-80, 1991 (PubMed).

Martin, Horwich, Hartl: "Prevention of protein denaturation under heat stress by the chaperonin Hsp60." in: Science (New York, N.Y.), Vol. 258, Issue 5084, pp. 995-8, 1992 (PubMed).

Georgopoulos, Hohn: "Identification of a host protein necessary for bacteriophage morphogenesis (the groE gene product)." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 75, Issue 1, pp. 131-5, 1978 (PubMed).

Catalog No. ABIN121249

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