TANK-Binding Kinase 1 (TBK1) antibody

Antigen: TANK-Binding Kinase 1 (TBK1)

Synonyms: NAK, T2K, FLJ11330, AI462036, AW048562, MGC150301, MGC150302, 1200008B05Rik, tbk1, MGC81514, TBK1, wu:fk70c05, zgc:136548, nak, t2k

Clonality: Monoclonal (108A429)

Host: Mouse

Reactivity: Cow (Bovine)

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Catalog no.: ABIN121250

Additional Information

Alternative name: TBK1

UniProt: Q9UHD2

Immunogen: Synthetic peptide corresponding to amino acids 563-577 of human TBK1 AA Sequence: YYQFKKDKAERRLAYC

Cross-Reactivity: Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Rat (Rattus)

Isotype: IgG1

Clone: 108A429

Description: NF-kB (nuclear factor kB) is sequestered in the cytoplasm by IkB family of inhibitory proteins that mask the nuclear localization signal of NF-kB thereby preventing translocation of NF-kB to the nucleus (1). External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Synthesis of IkBa is autoregulated (2). IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g (3-5). IKK1/a and IKK2/b are phosphorylated by NF-kB-inducing kinase (NIK) (6) and MAP kinase kinase kinase-1 (MEKK1) (7), respectively. Recently, a new IKK family member, TBK1 [(TANK-binding kinase), also known as NAK and T2K] has been identified (8). TANK (TRAF-associated NF-kB activator) interacts with TBK1 and activates NF-kB in a kinase dependent manner. Alternate Names: TANK-binding kinase 1.

Specificity: ABIN121250 recognizes TBK1. This sequence is 100% conserved between human, mouse, rat, dog and cow

Application Details

Application Notes: Western blot analysis: 1-2 ug/ml (an 80 kDa band should be observed). Recommended Positive Control: NIH 3T3 or RAW. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.5 mg/ml

Purification: Purified

Buffer: PBS containing 0.05% BSA as stabilizer and 0.05% Sodium Azide as preservative.

Storage: Store the antibody (undiluted) at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.

Restrictions: For Research Use only

Images

Western blot analysis of TBK1 using ABIN121250 at 2 ug/ml dilution against 10 ug/lane of lysate from 293 cells untransfected (lane 1) and transfected with human TBK1 cDNA (lane 2).

Publications

Verma, Stevenson, Schwarz et al.: "Rel/NF-kappa B/I kappa B family: intimate tales of association and dissociation." in: Genes & development, Vol. 9, Issue 22, pp. 2723-35, 1995 (PubMed).

Lee, Hagler, Chen et al.: "Activation of the IkappaB alpha kinase complex by MEKK1, a kinase of the JNK pathway." in: Cell, Vol. 88, Issue 2, pp. 213-22, 1997 (PubMed).

Malinin, Boldin, Kovalenko et al.: "MAP3K-related kinase involved in NF-kappaB induction by TNF, CD95 and IL-1." in: Nature, Vol. 385, Issue 6616, pp. 540-4, 1997 (PubMed).

Régnier, Song, Gao et al.: "Identification and characterization of an IkappaB kinase." in: Cell, Vol. 90, Issue 2, pp. 373-83, 1997 (PubMed).

DiDonato, Hayakawa, Rothwarf et al.: "A cytokine-responsive IkappaB kinase that activates the transcription factor NF-kappaB." in: Nature, Vol. 388, Issue 6642, pp. 548-54, 1997 (PubMed).

Verma, Stevenson: "IkappaB kinase: beginning, not the end." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 22, pp. 11758-60, 1997 (PubMed).

Mercurio, Zhu, Murray et al.: "IKK-1 and IKK-2: cytokine-activated IkappaB kinases essential for NF-kappaB activation." in: Science (New York, N.Y.), Vol. 278, Issue 5339, pp. 860-6, 1997 (PubMed).