Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase gamma (IKBKG) antibody

Antigen: Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase gamma (IKBKG)

Synonyms: IP, IP1, IP2, FIP3, IPD2, NEMO, FIP-3, Fip3p, AMCBX1, IKK-gamma, IKK[g], AI848108, AI851264, AW124339, 1110037D23Rik, Nemo, nemo, fj33c07, wu:fj33c07, zgc:113492, MGC97885, IKBKG, IKBKG_1, IKBKG_2, DmIKK-gamma, DmIKKgamma, IKKg, IKKgamma, Kenny, Key, dIKK, dIKK-gamma, DmelCG16910, CG16910

Clonality: Monoclonal (72C627)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Catalog no.: ABIN121252

Additional Information

Alternative name: NEMO / IKBKG

UniProt: Q9Y6K9

Immunogen: His-tagged full-length human IKK3/IKKg protein

Cross-Reactivity: Human, Mouse (Murine)

Isotype: IgG1

Clone: 72C627

Specificity: This antibody is specific for I Kappa K gamma.

Synonyms: IP, IP1, IP2, FIP3, IPD2, NEMO, FIP-3, Fip3p, AMCBX1, IKK-gamma, IKK[g], AI848108, AI851264, AW124339, 1110037D23Rik, Nemo, nemo, fj33c07, wu:fj33c07, zgc:113492, MGC97885, IKBKG, IKBKG_1, IKBKG_2, DmIKK-gamma, DmIKKgamma, IKKg, IKKgamma, Kenny, Key, dIKK, dIKK-gamma, DmelCG16910, CG16910

Application Details

Application Notes: Western blot analysis: 2.0 ug/ml, positive control: Jurkat or NIH 3T3 (a 48 kDa band is observed). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.5 mg/ml

Purification: Purified

Buffer: PBS containing 0.05 % BSA and 0.05 % sodium azide

Storage: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.

Restrictions: For Research Use only

Images

Western blot analysis of 30 ug of total cell lysate from Jurkat (lane 1) and NIH-3T3 (lane 2) cells with ABIN121252 at 2 ug/ml dilution.

Publications

Verma, Stevenson, Schwarz et al.: "Rel/NF-kappa B/I kappa B family: intimate tales of association and dissociation." in: Genes & development, Vol. 9, Issue 22, pp. 2723-35, 1995 (PubMed).

Régnier, Song, Gao et al.: "Identification and characterization of an IkappaB kinase." in: Cell, Vol. 90, Issue 2, pp. 373-83, 1997 (PubMed).

DiDonato, Hayakawa, Rothwarf et al.: "A cytokine-responsive IkappaB kinase that activates the transcription factor NF-kappaB." in: Nature, Vol. 388, Issue 6642, pp. 548-54, 1997 (PubMed).

Verma, Stevenson: "IkappaB kinase: beginning, not the end." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 22, pp. 11758-60, 1997 (PubMed).

Mercurio, Zhu, Murray et al.: "IKK-1 and IKK-2: cytokine-activated IkappaB kinases essential for NF-kappaB activation." in: Science (New York, N.Y.), Vol. 278, Issue 5339, pp. 860-6, 1997 (PubMed).

Yamaoka, Courtois, Bessia et al.: "Complementation cloning of NEMO, a component of the IkappaB kinase complex essential for NF-kappaB activation." in: Cell, Vol. 93, Issue 7, pp. 1231-40, 1998 (PubMed).

Jin, Giordano, Kibler et al.: "Role of adapter function in oncoprotein-mediated activation of NF-kappaB. Human T-cell leukemia virus type I Tax interacts directly with IkappaB kinase gamma." in: The Journal of biological chemistry, Vol. 274, Issue 25, pp. 17402-5, 1999 (PubMed).

Harhaj, Sun: "IKKgamma serves as a docking subunit of the IkappaB kinase (IKK) and mediates interaction of IKK with the human T-cell leukemia virus Tax protein." in: The Journal of biological chemistry, Vol. 274, Issue 33, pp. 22911-4, 1999 (PubMed).