Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase gamma (IKBKG) antibody

Details for Product No. ABIN121253
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Antigen
Reactivity
Human
(222), (48), (45), (25), (14), (2), (2), (2), (1)
Host
Mouse
(188), (35), (2), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(8), (8), (8), (5), (5), (5), (5), (5), (5), (5), (5), (3), (3), (3)
Application
Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(168), (97), (50), (47), (38), (24), (23), (22), (7), (3), (2), (1)
Pubmed 7 references available
Quantity 0.1 mg
Shipping to United States (Change)
Availability Discontinued
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Catalog No. ABIN121253
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Immunogen His-tagged full-length human IKK3/IKKg protein
Clone 46B844
Isotype IgG1
Purification Protein G Chromatography
Alternative Name NEMO / IKBKG
Background NF-kB (nuclear factor kB) is sequestered in the cytoplasm by IkB family of inhibitory proteins that mask the nuclear localization signal of NF-kB thereby preventing translocation of NF-kB to the nucleus (1). External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Synthesis of IkBa is autoregulated (2). IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g (3-7). IKK3/g preferentially interacts with IKK2/b and is required for activation of IKK complex. IKK3/g is also known as NEMO (NF-kB Essential MOdulator) (7). Recent data suggest that the human T-cell leukemia virus type I Tax oncoprotein that activates NF-kB binds neither to IKKa nor IKKb, but complexes directly with IKKg (8,9). This suggests that IKKg may be a key molecule acting as an adapter for onco-protein specific signaling to IKKa and IKKb (8).
UniProt Q9Y6K9
Application Notes Western blot analysis: 2.0 ug/ml, positive control: Daudi or Jurkat (48 kDa band is observed). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS containing 0.2% Gelatin, 0.05% Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.
Expiry Date 12 months
Supplier Images
anti-Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase gamma (IKBKG) antibody Western blot analysis of 30 ug of total cell lysate from Jurkat cells with ABIN121253 at 2 ug/ml dilution.
General Verma, Stevenson, Schwarz et al.: "Rel/NF-kappa B/I kappa B family: intimate tales of association and dissociation." in: Genes & development, Vol. 9, Issue 22, pp. 2723-35, 1995 (PubMed).

Régnier, Song, Gao et al.: "Identification and characterization of an IkappaB kinase." in: Cell, Vol. 90, Issue 2, pp. 373-83, 1997 (PubMed).

DiDonato, Hayakawa, Rothwarf et al.: "A cytokine-responsive IkappaB kinase that activates the transcription factor NF-kappaB." in: Nature, Vol. 388, Issue 6642, pp. 548-54, 1997 (PubMed).

Verma, Stevenson: "IkappaB kinase: beginning, not the end." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 22, pp. 11758-60, 1997 (PubMed).

Mercurio, Zhu, Murray et al.: "IKK-1 and IKK-2: cytokine-activated IkappaB kinases essential for NF-kappaB activation." in: Science (New York, N.Y.), Vol. 278, Issue 5339, pp. 860-6, 1997 (PubMed).

Yamaoka, Courtois, Bessia et al.: "Complementation cloning of NEMO, a component of the IkappaB kinase complex essential for NF-kappaB activation." in: Cell, Vol. 93, Issue 7, pp. 1231-40, 1998 (PubMed).

Geiss, Jin, Guo et al.: "A comprehensive view of regulation of gene expression by double-stranded RNA-mediated cell signaling." in: The Journal of biological chemistry, Vol. 276, Issue 32, pp. 30178-82, 2001 (PubMed).

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