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Interleukin 10 (IL10) antibody

Antigen

Interleukin 10 (IL10)

Synonyms CSIF, TGIF, IL-10, IL10A, MGC126450, MGC126451
Clonality Monoclonal (JES5-16E3)
Host
Reactivity
Alternatives

Mouse (Murine)

Application
Alternatives Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Western Blotting (WB)
4 references available
Catalog no. ABIN121256
Quantity 0.1 mg  (0.5 mg/ml)  (Variants)
Price 215.00 $   Plus shipping costs $35.00
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Availability Ships within 7 to 10 Business Days

Additional Information

Alternative name Interleukin-10 / IL10
Swiss-Prot P18893
Immunogen Recombinant mouse IL-10 expressed in E. coli
Isotype IgG2b  (Matching secondary antibodies)
Clone JES5-16E3
Specificity This antibody is specific for IL-10.

Application Details

Application Notes Western blot analysis. Flow Cytometry (Intracellular): please see reference (4) for details. ELISA: please see reference (1,2) for details. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Concentration 0.5 mg/ml
Buffer PBS containing 0.02% sodium azide
Storage Store undiluted at 2 - 8°C. Do not freeze. Shelf Life: six months from despatch.
Restrictions For Research Use only

Publications

Publications Guesdon, Ternynck, Avrameas: "The use of avidin-biotin interaction in immunoenzymatic techniques." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 27, Issue 8, pp. 1131-9, 1979 (PubMed).

Litton, Sander, Murphy et al.: "Early expression of cytokines in lymph nodes after treatment in vivo with Staphylococcus enterotoxin B." in: Journal of immunological methods, Vol. 175, Issue 1, pp. 47-58, 1994 (PubMed).

Sander, Hoeiduen, Andersson et al.: "Similar frequencies and kinetics of cytokine producing cells in murine peripheral blood and spleen. Cytokine detection by immunoassay and intracellular immunostaining." in: Journal of immunological methods, Vol. 166, Issue 2, pp. 201-14, 1994 (PubMed).

Prussin, Metcalfe: "Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies." in: Journal of immunological methods, Vol. 188, Issue 1, pp. 117-28, 1996 (PubMed).