Interleukin 25 (IL25) antibody

Antigen: Interleukin 25 (IL25)

Synonyms: IL-25, IL17E, IL-17E, Il17e, IL-17e, IL25, RGD1561632

Clonality: Monoclonal (68C1039-2)

Host: Mouse

Reactivity: Human, Mouse (Murine)

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Catalog no.: ABIN121259

Additional Information

Alternative name: Interleukin-25 / IL25

UniProt: Q9H293

Immunogen: Synthetic peptide of amino acids 115-132, obtained from the human IL-17E protein sequence

Cross-Reactivity: Human, Mouse (Murine)

Isotype: IgG1

Clone: 68C1039-2

Description: Recently, a number of cytokines belonging to the interleukin (IL)-17 family have been identified. These are termed as IL-17B, IL-17C (1) and IL-17E (2). IL-17 is a potent proinflammatory cytokine that plays roles in a number of diseases including rheumatoid arthritis (3), multiple sclerosis (4), and promotion of tumor growth (5). IL-17B, C, and E like IL-17 are able to induce proinflammatory responses. However, they do not bind to the IL-17 receptor suggesting that additional IL-17R related receptor might exist. Receptor for IL-17B and IL-17E has been independently isolated by Shi, et al (6) and Lee, et al. (2) and has been designated as EV127 (in mouse) and IL-17Rh1 (in human), respectively. IL-17E induces activation of NF-kB pathway and like IL-17 also induces production of IL-8.

Application Details

Application Notes: Western blot analysis: 2.0 ug/ml. Positive control: mouse testes (a 16 kDa band is observed). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.5 mg/ml

Purification: Purified

Buffer: PBS containing 0.05 % sodium azide

Storage: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.

Restrictions: For Research Use only

Images

Western blot analysis of IL-17E in mouse testis lysate using ABIN121259. Ten microgram of lysate was used per lane.

Publications

Shalom-Barak, Quach, Lotz: "Interleukin-17-induced gene expression in articular chondrocytes is associated with activation of mitogen-activated protein kinases and NF-kappaB." in: The Journal of biological chemistry, Vol. 273, Issue 42, pp. 27467-73, 1998 (PubMed).

Matusevicius, Kivisaekk, He et al.: "Interleukin-17 mRNA expression in blood and CSF mononuclear cells is augmented in multiple sclerosis." in: Multiple sclerosis (Houndmills, Basingstoke, England), Vol. 5, Issue 2, pp. 101-4, 1999 (PubMed).

Tartour, Fossiez, Joyeux et al.: "Interleukin 17, a T-cell-derived cytokine, promotes tumorigenicity of human cervical tumors in nude mice." in: Cancer research, Vol. 59, Issue 15, pp. 3698-704, 1999 (PubMed).

Li, Chen, Huang et al.: "Cloning and characterization of IL-17B and IL-17C, two new members of the IL-17 cytokine family." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, Issue 2, pp. 773-8, 2000 (PubMed).

Shi, Ullrich, Zhang et al.: "A novel cytokine receptor-ligand pair. Identification, molecular characterization, and in vivo immunomodulatory activity." in: The Journal of biological chemistry, Vol. 275, Issue 25, pp. 19167-76, 2000 (PubMed).

Lee, Ho, Maruoka et al.: "IL-17E, a novel proinflammatory ligand for the IL-17 receptor homolog IL-17Rh1." in: The Journal of biological chemistry, Vol. 276, Issue 2, pp. 1660-4, 2001 (PubMed).