NFKB Inhibitor Interacting Ras-Like 1 (NKIRAS1) antibody

Antigen: NFKB Inhibitor Interacting Ras-Like 1 (NKIRAS1)

Synonyms: KBRAS1, kappaB-Ras1, 2400004O09Rik, NKIRAS1, MGC92823, zgc:92823, nkiras1

Clonality: Monoclonal (184C278)

Host: Mouse

Reactivity: Human

Application: Western Blotting (WB)

Catalog no.: ABIN121266

Additional Information

Alternative name: NKIRAS1

UniProt: Q9NYS0

Cross-Reactivity: Human

Isotype: IgG1

Clone: 184C278

Description: NF-kB is silenced in the cytoplasm by an inhibitory protein, IkB (1). Synthesis of IkBa is autoregulated (2). IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g (3-6). External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Six members of IkB family members have been identified (1). One of the first genes induced following NF-kB activation is IkBa. Recently, two proteins, kB-Ras1 and kB-Ras2 have been identified (7). These two proteins interact with PEST domains of IkBa and IkBb and decrease their rate of degradation.

Specificity: This antibody is specific for k B-Ras1.

Application Details

Application Notes: Western blot: 0.5-1 ug/ml. Recommended Positive Control: 293 cell lysate. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.5 mg/ml

Purification: Purified

Buffer: PBS containing 0.02 % sodium azide

Storage: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.

Research Area: Signaling

Restrictions: For Research Use only

Images

Western Blot analysis of kB-Ras1 on 293 cell lysate using ABIN121266 at 0.5 ug/ml.

Publications

Verma, Stevenson, Schwarz et al.: "Rel/NF-kappa B/I kappa B family: intimate tales of association and dissociation." in: Genes & development, Vol. 9, Issue 22, pp. 2723-35, 1995 (PubMed).

Régnier, Song, Gao et al.: "Identification and characterization of an IkappaB kinase." in: Cell, Vol. 90, Issue 2, pp. 373-83, 1997 (PubMed).

DiDonato, Hayakawa, Rothwarf et al.: "A cytokine-responsive IkappaB kinase that activates the transcription factor NF-kappaB." in: Nature, Vol. 388, Issue 6642, pp. 548-54, 1997 (PubMed).

Verma, Stevenson: "IkappaB kinase: beginning, not the end." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 22, pp. 11758-60, 1997 (PubMed).

Mercurio, Zhu, Murray et al.: "IKK-1 and IKK-2: cytokine-activated IkappaB kinases essential for NF-kappaB activation." in: Science (New York, N.Y.), Vol. 278, Issue 5339, pp. 860-6, 1997 (PubMed).

Yamaoka, Courtois, Bessia et al.: "Complementation cloning of NEMO, a component of the IkappaB kinase complex essential for NF-kappaB activation." in: Cell, Vol. 93, Issue 7, pp. 1231-40, 1998 (PubMed).