Baculoviral IAP Repeat-Containing 7 (BIRC7) antibody

Antigen: Baculoviral IAP Repeat-Containing 7 (BIRC7)

Synonyms: KIAP, LIVIN, MLIAP, RNF50, ML-IAP, TIAP2, RGD1562883, BIRC7, zgc:165605, XLX, xEIAP, EIAP/XLX, Livin, E130019N06

Clonality: Monoclonal (88C570)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)

Catalog no.: ABIN121268

Additional Information

Alternative name: BIRC7 / LIVIN

UniProt: Q96CA5

Immunogen: Recombinant human livin protein

Cross-Reactivity: Human

Isotype: IgG1

Clone: 88C570

Description: The inhibitor of apoptosis (IAP) family of proteins regulates programmed cell death triggered by various stimuli. All IAPs have at least one baculovirus IAP repeat (BIR) motif that is essential for their anti-apoptotic activity. Recently, Kasof and Gomes (1), Vucic et al. (2) and Ashhab et al. (3) have identified a novel human inhibitor of apoptosis protein (IAP) family member termed Livin or ML-IAP, which contains a single baculoviral IAP repeat (BIR) domain and a COOH-terminal RING finger domain. Livin gene has two splicing variants that contain open reading frames of 298 and 280 amino acids and both contained a single copy of baculovirus IAP repeat (BIR) and RING domain. Both of the isoforms inhibit TNFa induced apoptosis in Jurkat cells. Livin expression is low in adult tissues. It is relatively expressed at a higher level in developmental tissues and in many cancer cells. Alternate Names: BIRC7, KIAP.

Application Details

Application Notes: Western blot analysis: 1-2 ug/ml. IF/ICC: 2-5 ug/ml. Recommended Positive Control: U266, HeLa, Jurkat. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.5 mg/ml

Purification: Purified

Buffer: PBS containing 0.05% BSA and 0.05% sodium azide

Storage: Store the antibody at 4-8°C for one month or at -20°C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.

Restrictions: For Research Use only

Images

Western blot detection of Livin in cell lysate transfected with human Livin cDNA. A protein band of apprximate molecular weight of 31 kDa is detected using ABIN121295 at 2 ug/ml dilution.

Publications

Kasof, Gomes: "Livin, a novel inhibitor of apoptosis protein family member." in: The Journal of biological chemistry, Vol. 276, Issue 5, pp. 3238-46, 2001 (PubMed).

Vucic, Stennicke, Pisabarro et al.: "ML-IAP, a novel inhibitor of apoptosis that is preferentially expressed in human melanomas." in: Current biology : CB, Vol. 10, Issue 21, pp. 1359-66, 2000 (PubMed).

Ashhab, Alian, Polliack et al.: "Two splicing variants of a new inhibitor of apoptosis gene with different biological properties and tissue distribution pattern." in: FEBS letters, Vol. 495, Issue 1-2, pp. 56-60, 2001 (PubMed).