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Details for Product No. ABIN121269

Methyl-CpG Binding Domain Protein 1 (MBD1) (AA 391-405) antibody

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Antigen
Synonyms RFT, PCM1, CXXC3, Cxxc3
Epitope
»Alternatives AA 391-405
Reactivity
»Alternatives Human
Host
»Alternatives Mouse
Clonality (Clone) Monoclonal ()
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives Western Blotting (WB)
Pubmed 3 references available
Catalog no. ABIN121269
Quantity 0.1 mg
Price
445.50 $   Plus shipping costs $45.00
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Availability Discontinued
Immunogen Synthetic peptide corresponding to amino acids 391-405 of human MBD1 AA Sequence:SESEDGAGSPPPYRR
Clone 100B272
Isotype IgG1
Purification Protein G chromatography
Alternative Name MBD1
Background DNA methylation, or the addition of methyl groups to cytosine bases in the dinucleotide CpG, is imperative to proper development and regulates gene expression. The methylation pattern involves the enzymatic processes of methylation and demethylation. The demethylation enzyme was recently found to be a mammalian protein, which exhibits demethylase activity associated to a methyl-CpG-binding domain (MBD) (1). The enzyme is able to revert methylated cytosine bases to cytosines within the particular dinucleotide sequence mdCpdG by catalyzing the cleaving of the methyl group as methanol. MeCP2 and MBD1 (PCM1) are first found to repress transcription by binding specifically to methylated DNA (2). MBD2 and MBD4 (also known as MED1) were later found to colocalize with foci of heavily methylated satellite DNA and believed to mediate the biological functions of the methylation signal. Surprisingly, MBD3 does not bind methylated DNA both in vivo and in vitro. MBD1, MBD2, MBD3, and MBD4 are found to be expressed in somatic tissues, but the expression of MBD1 and MBD2 is reduced or absent in embryonic stem cells, which are known to be deficient in MeCP1 activity. MBD4 have homology to bacterial base excision repair DNA N-glycosylases/lyases (3). In some microsatellite unstable tumors MBD4 is mutated at an exonic polynucleotide tract (4).
UniProt Q9UIS9
Research Area Chromatin and Nuclear Signaling
Application Notes Western blot analysis: 2 ug/ml.Recommended positive control: HeLa (a 70 kDa band is observed). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS containing 0.02 % sodium azide
Preservative Sodium azide
Storage 4 °C/-20 °C
Storage Comment Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.
Expiry Date 12 months
Hendrich, Bird: "Identification and characterization of a family of mammalian methyl-CpG binding proteins." in: Molecular and cellular biology, Vol. 18, Issue 11, pp. 6538-47, 1998 (PubMed).

Petronzelli, Riccio, Markham et al.: "Biphasic kinetics of the human DNA repair protein MED1 (MBD4), a mismatch-specific DNA N-glycosylase." in: The Journal of biological chemistry, Vol. 275, Issue 42, pp. 32422-9, 2000 (PubMed).

Walterfang, Yucel, Walker et al.: "Adolescent obsessive compulsive disorder heralding chorea-acanthocytosis." in: Movement disorders : official journal of the Movement Disorder Society, Vol. 23, Issue 3, pp. 422-5, 2008 (PubMed).

Alternatives for antigen "Methyl-CpG Binding Domain Protein 1 (MBD1)", type "Antibodies"
Hosts (39), (15)
Reactivities (52), (18), (16), (2), (2)
Applications (44), (16), (10), (6), (3), (2), (1), (1), (1)
Conjugates (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (7), (5), (3), (2), (2), (2), (2), (1), (1), (1)
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