Methyl-CpG Binding Domain Protein 3 (MBD3) antibody

Antigen: Methyl-CpG Binding Domain Protein 3 (MBD3)

Synonyms: AI181826, AU019209, xmbd3, MGC69548, MBD3, DKFZp459N1635

Clonality: Monoclonal (106B691)

Host: Mouse

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Application: Western Blotting (WB)

Catalog no.: ABIN121270

Additional Information

Alternative name: MBD3

UniProt: O95983

Immunogen: Amino acids 215-230 of human MBD3 protein AA Sequence:CKAFMVTDEDIRKQEE

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus)

Isotype: IgG1

Clone: 106B691

Description: DNA methylation, or the addition of methyl groups to cytosine bases in the dinucleotide CpG, is imperative to proper development and regulates gene expression. The methylation pattern involves the enzymatic processes of methylation and demethylation. The demethylation enzyme was recently found to be a mammalian protein, which exhibits demethylase activity associated to a methyl-CpG-binding domain (MBD) (4). The enzyme is able to revert methylated cytosine bases to cytosines within the particular dinucleotide sequence mdCpdG by catalyzing the cleaving of the methyl group as methanol. MeCP2 and MBD1 (PCM1) are first found to repress transcription by binding specifically to methylated DNA (3). MBD2 and MBD4 (also known as MED1) were later found to colocalize with foci of heavily methylated satellite DNA and believed to mediate the biological functions of the methylation signal. Surprisingly, MBD3 does not bind methylated DNA both in vivo and in vitro. MBD1, MBD2, MBD3, and MBD4 are found to be expressed in somatic tissues, but the expression of MBD1 and MBD2 is reduced or absent in embryonic stem cells, which are known to be deficient in MeCP1 activity. MBD4 have homology to bacterial base excision repair DNA N-glycosylases/lyases (2). In some microsatellite unstable tumors MBD4 is mutated at an exonic polynucleotide tract (1).

Application Details

Application Notes: Western blot: 2 ug/ml. Recommended Positive Control : HeLa (nuclear fraction). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.5 mg/ml

Purification: Purified

Buffer: PBS containing 0.02 % sodium azide

Storage: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.

Research Area: Chromatin and Nuclear Signaling

Restrictions: For Research Use only

Images

Western blot analysis of MBD3 in HeLa cell lysate with anti-MBD3 McAb (Cat. #. ABIN121270. A protein band of approximate molecular weight of 50 kDa is detected

Publications

Hendrich, Bird: "Identification and characterization of a family of mammalian methyl-CpG binding proteins." in: Molecular and cellular biology, Vol. 18, Issue 11, pp. 6538-47, 1998 (PubMed).

Balmer, Arredondo, Samaco et al.: "MECP2 mutations in Rett syndrome adversely affect lymphocyte growth, but do not affect imprinted gene expression in blood or brain." in: Human genetics, Vol. 110, Issue 6, pp. 545-52, 2002 (PubMed).