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Details for Product No. ABIN121272

Microphthalmia-Associated Transcription Factor (MITF) (AA 301-312) antibody

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Antigen
Synonyms CMM8, MI, WS2, WS2A, bHLHe32, BCC2, Bhlhe32, Gsfbcc2, Vitiligo, Wh, bw, mi, vit, CMI9, MITF-H, MITF-M, MITF, ws2a, mitfa, MITF-A, LOC100313655, mitfb
Epitope
»Alternatives AA 301-312
Reactivity
»Alternatives Human, Mouse (Murine), Rat (Rattus)
Host
»Alternatives Mouse
Clonality (Clone) Monoclonal ()
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives Western Blotting (WB)
Pubmed 3 references available
Catalog no. ABIN121272
Quantity 0.1 mg
Price
445.50 $   Plus shipping costs $45.00
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Availability Will be delivered in 6 to 8 Business Days
Immunogen Synthetic peptide corresponding to amino acids 301-312 of Mitf AA Sequence:PSTGLCSPDLVN
Clone 21D1418
Isotype IgG1
Purification Protein G Chromatography
Alternative Name MITF
Background Microphthalmia-associated bHLH-LZ transcription factor (Mitf) is a positively acting transcription factor containing a transcription activation domain and a bHLH-LZ domain that mediates DNA-binding and dimerization. Both the transcription activation and bHLH-LZ domains are highly conserved in the closely related TFE3, TFEB and TFEC transcription factors. Mitf can form heterodimers in vitro with the above transcription factors, but not with other bHLH-LZ factors such as Myc, Max or USF. Mitf is expressed as multiple isoforms, which share common transcription activation and DNA-binding domains, but differ in the amino-terminal region. Mitf is targeted by three kinases: GSK-3beta, Map kinase ERK2 and members of the p90 Rsk family of kinases.
UniProt O75030
Research Area Transcription Factors
Application Notes Western blot analysis: 3-5 ug/ml. Recommended positive control: A375 or RAW. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: Mitf produces, by alternative splicing, 10 different transcripts, all with introns, putatively encoding 10 different protein isoforms. Therefore, observed molecular weights may vary depending on MITF isoforms expressed.
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS containing 0.05% Sodium Azide as preservative and 0.2% Gelatin as stabilizer
Preservative Sodium azide
Storage 4 °C/-20 °C
Storage Comment Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.
Expiry Date 12 months
Udono, Yasumoto, Takeda et al.: "Structural organization of the human microphthalmia-associated transcription factor gene containing four alternative promoters." in: Biochimica et biophysica acta, Vol. 1491, Issue 1-3, pp. 205-19, 2000 (PubMed).

Hallsson, Favor, Hodgkinson et al.: "Genomic, transcriptional and mutational analysis of the mouse microphthalmia locus." in: Genetics, Vol. 155, Issue 1, pp. 291-300, 2000 (PubMed).

Goding: "Mitf from neural crest to melanoma: signal transduction and transcription in the melanocyte lineage." in: Genes & development, Vol. 14, Issue 14, pp. 1712-28, 2000 (PubMed).

Alternatives for antigen "Microphthalmia-Associated Transcription Factor (MITF)", type "Antibodies"
Hosts (59), (39), (2)
Reactivities (95), (45), (24), (16), (15), (14), (3), (2), (2)
Applications (67), (31), (23), (12), (12), (10), (10), (8), (6), (5), (4), (3), (2), (1), (1)
Conjugates (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (17), (9), (6), (6), (4), (2), (2), (2), (2), (1), (1), (1), (1)
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