anti-MITF antibody (Microphthalmia-Associated Transcription Factor) (AA 301-312)

Details for Product anti-MITF Antibody No. ABIN121272
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AA 301-312
(39), (16), (12), (11), (7), (5), (4), (4), (4), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Human, Mouse (Murine), Rat (Rattus)
(129), (55), (28), (4), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1)
(90), (37), (2), (2)
Clonality (Clone)
Monoclonal ()
This MITF antibody is un-conjugated
(3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Western Blotting (WB)
(105), (44), (27), (20), (19), (18), (16), (11), (10), (8), (7), (3), (3), (3), (2), (1)
Pubmed 3 references available
Quantity 0.1 mg
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Immunogen Synthetic peptide corresponding to amino acids 301-312 of Mitf AA Sequence:PSTGLCSPDLVN
Clone 21D1418
Isotype IgG1
Purification Protein G Chromatography
Alternative Name MITF (MITF Antibody Abstract)
Background Microphthalmia-associated bHLH-LZ transcription factor (Mitf) is a positively acting transcription factor containing a transcription activation domain and a bHLH-LZ domain that mediates DNA-binding and dimerization. Both the transcription activation and bHLH-LZ domains are highly conserved in the closely related TFE3, TFEB and TFEC transcription factors. Mitf can form heterodimers in vitro with the above transcription factors, but not with other bHLH-LZ factors such as Myc, Max or USF. Mitf is expressed as multiple isoforms, which share common transcription activation and DNA-binding domains, but differ in the amino-terminal region. Mitf is targeted by three kinases: GSK-3beta, Map kinase ERK2 and members of the p90 Rsk family of kinases.
UniProt O75030
Research Area Transcription Factors
Application Notes Western blot analysis: 3-5 ug/ml. Recommended positive control: A375 or RAW. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: Mitf produces, by alternative splicing, 10 different transcripts, all with introns, putatively encoding 10 different protein isoforms. Therefore, observed molecular weights may vary depending on MITF isoforms expressed.
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS containing 0.05% Sodium Azide as preservative and 0.2% Gelatin as stabilizer
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.
Expiry Date 12 months
Supplier Images
Western Blotting (WB) image for anti-MITF antibody (Microphthalmia-Associated Transcription Factor) (AA 301-312) (ABIN121272) Western blot analysis of Mitf using ABIN121272 at 1:1000 dilution against A375 (human...
General Goding: "Mitf from neural crest to melanoma: signal transduction and transcription in the melanocyte lineage." in: Genes & development, Vol. 14, Issue 14, pp. 1712-28, 2000 (PubMed).

Hallsson, Favor, Hodgkinson et al.: "Genomic, transcriptional and mutational analysis of the mouse microphthalmia locus." in: Genetics, Vol. 155, Issue 1, pp. 291-300, 2000 (PubMed).

Udono, Yasumoto, Takeda et al.: "Structural organization of the human microphthalmia-associated transcription factor gene containing four alternative promoters." in: Biochimica et biophysica acta, Vol. 1491, Issue 1-3, pp. 205-19, 2000 (PubMed).

Catalog No. ABIN121272
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