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MutL Homolog 1, Colon Cancer, Nonpolyposis Type 2 (E. Coli) (MLH1) (AA 387-403) antibody

Details for Product No. ABIN121273
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Antigen
Epitope
AA 387-403
(17), (8), (5), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(99), (38), (34), (14), (13), (7), (5), (1), (1), (1), (1), (1)
Host
Mouse
(73), (31)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(72), (30), (29), (17), (10), (10), (10), (9), (8), (6), (2)
Pubmed 1 reference available
Quantity 0.1 mg
Shipping to United States (Change)
Availability Discontinued
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Catalog No. ABIN121273
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Immunogen Synthetic peptide corresponding to amino acids 387-403 of human MLH1 GenBank no. NP_000240.1. AA Sequence:DSREQKLDAFLQPLSKP
Clone 164C819
Isotype IgG
Purification Protein G Chromatography.
Alternative Name MLH1
Background Human Mut L Homologue (MLH1) is homologous to the bacterial DNA mismatch repair Mult L gene. Mutations in the hMLH1 gene is associated with hereditary non-polyposis colon cancer and various other cancers. Alternate Names: MutL protein homolog 1, HNPCC.
UniProt P40692
Research Area Cancer, DNA/RNA
Application Notes Western blot: 2 - 3 ug/ml. In Molt-4, a 83 kDa band is observed. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS containing 0.05% BSA as stabilizer and 0.05% Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.
Expiry Date 12 months
Supplier Images
anti-MutL Homolog 1, Colon Cancer, Nonpolyposis Type 2 (E. Coli) (MLH1) (AA 387-403) antibody Western blot analysis of MLH-1 in Molt-4 cell lysate using ABIN121273 at 2 ug/ml, and exposed for 15 minutes.
General Bronner, Baker, Morrison et al.: "Mutation in the DNA mismatch repair gene homologue hMLH1 is associated with hereditary non-polyposis colon cancer." in: Nature, Vol. 368, Issue 6468, pp. 258-61, 1994 (PubMed).

Validation Images
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