MutL Homolog 1, Colon Cancer, Nonpolyposis Type 2 (E. Coli) (MLH1) antibody

Antigen: MutL Homolog 1, Colon Cancer, Nonpolyposis Type 2 (E. Coli) (MLH1)

Synonyms: FCC2, COCA2, HNPCC, hMLH1, HNPCC2, MGC5172, MGC66301, zgc:66301, MLH1, MGC140127, LOC100232198

Clonality: Monoclonal (164C819)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Catalog no.: ABIN121273

Additional Information

Alternative name: MLH1

UniProt: P40692

Immunogen: Synthetic peptide corresponding to amino acids 387-403 of human MLH1 GenBank no. NP_000240.1. AA Sequence:DSREQKLDAFLQPLSKP

Cross-Reactivity: Human

Isotype: IgG

Clone: 164C819

Description: Human Mut L Homologue (MLH1) is homologous to the bacterial DNA mismatch repair Mult L gene. Mutations in the hMLH1 gene is associated with hereditary non-polyposis colon cancer and various other cancers. Alternate Names: MutL protein homolog 1, HNPCC.

Specificity: The antibody reacts with MutL protein homolog 1. This peptide sequence is 93% homologous to mouse and dog MLH1

Application Details

Application Notes: Western blot: 2 - 3 ug/ml. In Molt-4, a 83 kDa band is observed. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.5 mg/ml

Purification: Purified

Buffer: PBS containing 0.05% BSA and 0.05% sodium azide

Storage: Store the antibody at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.

Research Area: Cancer, DNA/RNA

Restrictions: For Research Use only

Images

Western blot analysis of MLH-1 in Molt-4 cell lysate using ABIN121273 at 2 ug/ml, and exposed for 15 minutes.

Publications

Bronner, Baker, Morrison et al.: "Mutation in the DNA mismatch repair gene homologue hMLH1 is associated with hereditary non-polyposis colon cancer." in: Nature, Vol. 368, Issue 6468, pp. 258-61, 1994 (PubMed).