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HtrA Serine Peptidase 2 (HTRA2) (AA 335-350) antibody

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AA 335-350
(20), (16), (14), (9), (7), (4), (4), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
(136), (38), (34), (1), (1), (1)
(102), (34)
Clonality (Clone)
Monoclonal ()
(6), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(105), (68), (50), (20), (20), (11), (4), (4), (3), (2), (1), (1), (1), (1)
Pubmed 3 references available
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Quantity 0.1 mg
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Immunogen Synthetic peptide containing amino acids 335-350 of human Omi/HtrA2
Clone 196C429
Isotype IgG1
Purification Protein G chromatography
Alternative Name HTRA2 / PRSS25 (HTRA2 Antibody Abstract)
Background The inhibitor of apoptosis (IAP) family of proteins regulates programmed cell death triggered by various stimuli. All IAPs have at least one baculovirus IAP repeat (BIR) motif that is essential for their anti-apoptotic activity (1-3). Recently, a serine protease has been isolated, which is released from the mitochondria upon induction of apoptosis by apoptotic stimuli. This protein called Omi/HtrA2 is a mitochondrial protein that binds to IAP (4). Like Smac/DIABLO, the mature Omi protein contains a conserved IAP-binding motif (AVPS) at its N terminus. The deregulated expression of Omi in the cytoplasm of mammalian cells induces apoptosis indicating that Omi could participate in the mitochondrial apoptotic pathway.
UniProt O43464
Application Notes Western blot: 2 - 3 ug/ml. Immunohistochemistry on paraffin sections. Recommended Positive Control: Jurkat or HL-60 cell lysate. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS containing 0.05 % sodium azide and 0.2 % gelatin
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.
Expiry Date 12 months
Supplier Images
Western Blotting (WB) image for anti-HtrA Serine Peptidase 2 (HTRA2) (AA 335-350) antibody (ABIN121278) Western blot detection of Omi/HtrA2 in HL-60 cell lysate. Two protein bands of approx...
Image no. 2 for anti-HtrA Serine Peptidase 2 (HTRA2) (AA 335-350) antibody (ABIN121278) IHC testing(5) using ABIN121278 shows cytoplasmic staining in stomach tumor tissue (A...
General Hegde, Srinivasula, Zhang et al.: "Identification of Omi/HtrA2 as a mitochondrial apoptotic serine protease that disrupts inhibitor of apoptosis protein-caspase interaction." in: The Journal of biological chemistry, Vol. 277, Issue 1, pp. 432-8, 2002 (PubMed).

Reed, Bischoff: "BIRinging chromosomes through cell division--and survivin' the experience." in: Cell, Vol. 102, Issue 5, pp. 545-8, 2000 (PubMed).

Deveraux, Reed: "IAP family proteins--suppressors of apoptosis." in: Genes & development, Vol. 13, Issue 3, pp. 239-52, 1999 (PubMed).

Catalog No. ABIN121278

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