HtrA Serine Peptidase 2 (HTRA2) antibody

Antigen: HtrA Serine Peptidase 2 (HTRA2)

Synonyms: OMI, PARK13, PRSS25, Omi, mnd2, Prss25, AI481710, HTRA2

Clonality: Monoclonal (196C429)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN121278

Additional Information

Alternative name: HTRA2 / PRSS25

UniProt: O43464

Immunogen: Synthetic peptide containing amino acids 335-350 of human Omi/HtrA2

Cross-Reactivity: Human

Isotype: IgG1

Clone: 196C429

Description: The inhibitor of apoptosis (IAP) family of proteins regulates programmed cell death triggered by various stimuli. All IAPs have at least one baculovirus IAP repeat (BIR) motif that is essential for their anti-apoptotic activity (1-3). Recently, a serine protease has been isolated, which is released from the mitochondria upon induction of apoptosis by apoptotic stimuli. This protein called Omi/HtrA2 is a mitochondrial protein that binds to IAP (4). Like Smac/DIABLO, the mature Omi protein contains a conserved IAP-binding motif (AVPS) at its N terminus. The deregulated expression of Omi in the cytoplasm of mammalian cells induces apoptosis indicating that Omi could participate in the mitochondrial apoptotic pathway.

Application Details

Application Notes: Western blot: 2 - 3 ug/ml. Immunohistochemistry on paraffin sections. Recommended Positive Control: Jurkat or HL-60 cell lysate. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.5 mg/ml

Purification: Purified

Buffer: PBS containing 0.05 % sodium azide and 0.2 % gelatin

Storage: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.

Restrictions: For Research Use only

Images

Western blot detection of Omi/HtrA2 in HL-60 cell lysate. Two protein bands of approximate molecular weight of 50 and 38 kDa were detected using ABIN121278 at 3 ug/ml dilution. The 50 kDa and 38 kDa bands may represent precursor and mature form of Omi.

IHC testing(5) using ABIN121278 shows cytoplasmic staining in stomach tumor tissue (A) and very weak staining in normal stomach tissue (B).

Publications

Deveraux, Reed: "IAP family proteins--suppressors of apoptosis." in: Genes & development, Vol. 13, Issue 3, pp. 239-52, 1999 (PubMed).

Reed, Bischoff: "BIRinging chromosomes through cell division--and survivin' the experience." in: Cell, Vol. 102, Issue 5, pp. 545-8, 2000 (PubMed).

Hegde, Srinivasula, Zhang et al.: "Identification of Omi/HtrA2 as a mitochondrial apoptotic serine protease that disrupts inhibitor of apoptosis protein-caspase interaction." in: The Journal of biological chemistry, Vol. 277, Issue 1, pp. 432-8, 2002 (PubMed).