Tumor Protein P73 (TP73) (Isoform delta N) antibody

Antigen: Tumor Protein P73 (TP73)

Synonyms: TP73, P73, Trp73, tp73, p73, Tp73, TAp73, zp73theta

Binding Site: Isoform delta N

Clonality: Monoclonal (38C674)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB), Chromatin Immunoprecipitation (ChiP), Immunohistochemistry (IHC)

Catalog no.: ABIN121283

Additional Information

UniProt: O15350

Immunogen: Peptide corresponding to amino acid residues 2-13 of human dNp73. AA Sequence: LYVGDPARHLAT

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus)

Isotype: IgG1

Clone: 38C674

Description: P73 was identified as a long-lost cousin of tumor suppressor protein, p53 (1). p73 has high homology with p53 as well as with p63, a gene implicated in the maintenance of epithelial stem cells. Significant homology between p53, p63, and p73 (approximately 63% amino acid identity in the DNA-binding domain suggest that they may have overlapping functions in the regulation of gene expression (2). The targeted disruption of p73 gene leads to defects hippocampal dysgenesis, hydrocephalus, chronic inflammation and infections (3). Recently, spilicing variant mRNAs of p73 has been identified in MCF-7, a breast carcinoma cell line. These mRNAs code for variant p73 proteins bearing distinct carboxy-terminal structures suggesting that the carboxy- terminal region of p73 may be important for the functions of this protein (4). Recently, Yang et al. (2000) have cloned a p73 variant that lacks an amino-terminal transactivation domain, called deltaNp73 (5). The dNp73 message is transcribed from an alternative promoter located in intron 3. Alternate Names: Gamma Np73.

Specificity: This antibody is specific for p73 delta-N. It reacts with an epitope that is located on the N-terminal region of human gamma Np73 protein and does not cross reacts with full-lenth p73. Mouse and human sequences are more than 95 % identical at these amino acid residues. In mouse, alanine at position 8 is replaced by methionine

Application Details

Application Notes: Western blotting: 1-3 ug/ml. Immunohistochemistry on paraffin sections: 1/2000. ChIP: 5 ug. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.5 mg/ml

Purification: Purified

Buffer: PBS containing 0.05% BSA as stabilizer and 0.05% Sodium Azide as preservative.

Storage: Store the antibody (undiluted) at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.

Restrictions: For Research Use only

Images

Western blot analysis for p73N using ABIN121283 at 1 ug/ml dilution in a cell line transfected with p73N cDNA.

Publications

Kaghad, Bonnet, Yang et al.: "Monoallelically expressed gene related to p53 at 1p36, a region frequently deleted in neuroblastoma and other human cancers." in: Cell, Vol. 90, Issue 4, pp. 809-19, 1997 (PubMed).

Ueda, Hijikata, Takagi et al.: "New p73 variants with altered C-terminal structures have varied transcriptional activities." in: Oncogene, Vol. 18, Issue 35, pp. 4993-8, 1999 (PubMed).

Yang, Walker, Bronson et al.: "p73-deficient mice have neurological, pheromonal and inflammatory defects but lack spontaneous tumours." in: Nature, Vol. 404, Issue 6773, pp. 99-103, 2000 (PubMed).

Levrero, De Laurenzi, Costanzo et al.: "The p53/p63/p73 family of transcription factors: overlapping and distinct functions." in: Journal of cell science, Vol. 113 ( Pt 10), pp. 1661-70, 2000 (PubMed).