Tumor Necrosis Factor Receptor Superfamily, Member 11a, NFKB Activator (TNFRSF11A) antibody

Antigen: Tumor Necrosis Factor Receptor Superfamily, Member 11a, NFKB Activator (TNFRSF11A)

Synonyms: FEO, OFE, ODFR, OSTS, PDB2, RANK, CD265, OPTB7, TRANCER, LOH18CR1, TNFRSF11A

Clonality: Monoclonal (64C1385)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB)

Catalog no.: ABIN121294

Additional Information

Alternative name: CD265 / RANK

UniProt: Q9Y6Q6

Immunogen: Recombinant protein containing extracellular domain of human RANK

Cross-Reactivity: Human, Mouse (Murine)

Isotype: IgG1

Clone: 64C1385

Description: Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Apoptosis is induced by certain cytokines including TNF and Fas ligand of the TNF family through their death domain containing receptors, TNFR1 and Fas. Receptor activator of NF-kB (RANK) is a recently cloned member of the TNFR superfamily with no significant homology to other members of this family (1). RANK ligand (RANKL/TRANCE/ OPGL) binds to RANK on dendritic cells, upregulates the expression of anti- apoptotic protein BcL-XL suggesting a role in dendritic cell survival (2). The cytoplasmic domain of RANK interacts with TRAF2, TRAF5 and TRAF6 (3,4). Overexpression of RANK activates NF-kB and c-Jun-terminal kinase (JNK) pathways (3). Recent studies have shown that RANK interaction with TRAF6 activates NF-kB, whereas JNK activation is mediated through binding of RANK to TRAF2 (4).

Specificity: This antibody is specific for RANK.

Application Details

Application Notes: Western blotting (1-2 ug/ml). Immunoprecipitation (1-2 ug/ml). Flow Cytometry (5 ug for 10e6 cells). Recommended Positive Control: activated T cells or RAW cells (97-120 kDa band should be observed). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.5 mg/ml

Purity: Ig fraction

Purification: Purified

Buffer: PBS containing 0.2 % gelatin, 0.05 % sodium azide

Storage: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.

Restrictions: For Research Use only

Images

Detection of RANK in RAW cells. Lane 1. Western blot detection of RANK in RAW, a mouse cell line. Lane 2 & 3. IP/Western blot analysis of RANK. RANK protein from RAW cell lysate was immunoprecipitated either with control antibody (lane 2) or ABIN121294 (lane 3), the immunopricipitated proteins were resolved on SDS-PAGE. RANK was detected by western blot using ABIN121294 antibody.

Flow cytometry analysis of RANK using ABIN121294 at 5 ug/106 RAW cells.

Publications

Anderson, Maraskovsky, Billingsley et al.: "A homologue of the TNF receptor and its ligand enhance T-cell growth and dendritic-cell function." in: Nature, Vol. 390, Issue 6656, pp. 175-9, 1997 (PubMed).

Wong, Josien, Lee et al.: "TRANCE (tumor necrosis factor [TNF]-related activation-induced cytokine), a new TNF family member predominantly expressed in T cells, is a dendritic cell-specific survival factor." in: The Journal of experimental medicine, Vol. 186, Issue 12, pp. 2075-80, 1998 (PubMed).

Darnay, Haridas, Ni et al.: "Characterization of the intracellular domain of receptor activator of NF-kappaB (RANK). Interaction with tumor necrosis factor receptor-associated factors and activation of NF-kappab and c-Jun N-terminal kinase." in: The Journal of biological chemistry, Vol. 273, Issue 32, pp. 20551-5, 1998 (PubMed).

Darnay, Ni, Moore et al.: "Activation of NF-kappaB by RANK requires tumor necrosis factor receptor-associated factor (TRAF) 6 and NF-kappaB-inducing kinase. Identification of a novel TRAF6 interaction motif." in: The Journal of biological chemistry, Vol. 274, Issue 12, pp. 7724-31, 1999 (PubMed).