CD265 / RANK antibody

Antigen: CD265 / RANK

Clonality: Monoclonal (9A725)

Host: Mouse

Reactivity: Human, Mouse (Murine)

Conjugate: Un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN121295

Additional Information

Alternative name:

UniProt: Q9Y6Q6

Immunogen: Fusion protein containing Amino Acid residues 326-616 of human RANK

Cross-Reactivity: Human, Mouse (Murine)

Isotype: IgG1

Clone: 9A725

Description: Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Apoptosis is induced by certain cytokines including TNF and Fas ligand of the TNF family through their death domain containing receptors, TNFR1 and Fas. Receptor activator of NF-kB (RANK) is a recently cloned member of the TNFR superfamily with no significant homology to other members of this family (1). RANK ligand (RANKL/TRANCE/ OPGL) binds to RANK on dendritic cells, upregulates the expression of anti- apoptotic protein BcL-XL suggesting a role in dendritic cell survival (2). The cytoplasmic domain of RANK interacts with TRAF2, TRAF5 and TRAF6 (3,4). Overexpression of RANK activates NF-kB and c-Jun-terminal kinase (JNK) pathways (3). Recent studies have shown that RANK interaction with TRAF6 activates NF-kB, whereas JNK activation is mediated through binding of RANK to TRAF2 (4). Alternate Names: TNFRSF11A, Receptor Activator of NF-KB, Osteoclast Differentiation Factor receptor, ODFR, Tumor Necrosis Factor Receptor Superfamily Member 11A.

Application Details

Application Notes: Flow cytometric analysis on cell surface (6). Immunohistochemistry on Paraffin sections (Ref5, see also Protocol below). Western Blot (1-2 ug/ml): Raw 264 Cells can be used as positive control, detects a protein of approx. 97 kDa. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.5 mg/ml

Purification: Purified

Buffer: PBS containing 0.05% BSA as stabilizer and 0.05% Sodium Azide as preservative.

Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf Life: One year from despatch.

Restrictions: For Research Use only

Images

Western blot analysis of RANK in 293 cells transfected with RANK cDNA. A single protein band with an approximate mol. wt. of 97 kDa is detected.

Flow analysis of RANK in RAW cells using 2ug of ABIN121295. Shaded histogram represents RAW cells without antibody, green represents isotype control, purple represents anti-RANK antibody.

Publications

Anderson, Maraskovsky, Billingsley et al.: "A homologue of the TNF receptor and its ligand enhance T-cell growth and dendritic-cell function." in: Nature, Vol. 390, Issue 6656, pp. 175-9, 1997 (PubMed).

Wong, Josien, Lee et al.: "TRANCE (tumor necrosis factor [TNF]-related activation-induced cytokine), a new TNF family member predominantly expressed in T cells, is a dendritic cell-specific survival factor." in: The Journal of experimental medicine, Vol. 186, Issue 12, pp. 2075-80, 1998 (PubMed).

Darnay, Haridas, Ni et al.: "Characterization of the intracellular domain of receptor activator of NF-kappaB (RANK). Interaction with tumor necrosis factor receptor-associated factors and activation of NF-kappab and c-Jun N-terminal kinase." in: The Journal of biological chemistry, Vol. 273, Issue 32, pp. 20551-5, 1998 (PubMed).

Darnay, Ni, Moore et al.: "Activation of NF-kappaB by RANK requires tumor necrosis factor receptor-associated factor (TRAF) 6 and NF-kappaB-inducing kinase. Identification of a novel TRAF6 interaction motif." in: The Journal of biological chemistry, Vol. 274, Issue 12, pp. 7724-31, 1999 (PubMed).

Fiumara, Snell, Li et al.: "Functional expression of receptor activator of nuclear factor kappaB in Hodgkin disease cell lines." in: Blood, Vol. 98, Issue 9, pp. 2784-90, 2001 (PubMed).

Bharti, Takada, Shishodia et al.: "Evidence that receptor activator of nuclear factor (NF)-kappaB ligand can suppress cell proliferation and induce apoptosis through activation of a NF-kappaB-independent and TRAF6-dependent mechanism." in: The Journal of biological chemistry, Vol. 279, Issue 7, pp. 6065-76, 2004 (PubMed).