rho-Associated, Coiled-Coil Containing Protein Kinase 1 (ROCK1) (AA 1114-1126), (cleaved) antibody

Details for Product No. ABIN121298
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Antigen
Epitope
AA 1114-1126, cleaved
(24), (24), (12), (12), (9), (6), (4), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Dog (Canine), Human, Monkey, Mouse (Murine), Rabbit, Rat (Rattus)
(108), (63), (59), (3), (1), (1)
Host
Mouse
(95), (19)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3)
Application
Western Blotting (WB)
(82), (30), (29), (18), (14), (11), (10), (5), (1), (1), (1)
Pubmed 2 references available
Quantity 0.1 mg
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Immunogen Synthetic peptide sequence corresponding to amino acids 1114-1126 of human ROCK-1 AA Sequence:GNLPESRIEGWLS
Clone 154C1465
Isotype IgG
Purification Protein G Chromatography
Alternative Name ROCK1 (ROCK1 Antibody Abstract)
Background The Rho target protein, Rho-associated coiled coil-containing protein kinase (ROCK)-1 has been found to be a new Caspase-3 substrate. ROCK, one of the effectors of the small GTPase Rho, has recently been shown to contribute significantly to myosin light chain (MLC) activation through two pathways: direct phosphorylation of MLC and phosphorylation of MLC phosphatase, leading to its inhibition. The increase in cellular contractility that is necessary for apoptotic membrane blebbing implies sustained augmentation of MLC phosphorylation. ROCK-1 consists of an amino-terminal kinase domain and an inhibitory cysteine/histidine-rich C-terminal domain that is located within a pleckstrin-homology region. They are joined by a variable region that contains the Rho-binding domain. During apoptosis, ROCK-I is cleaved by caspase-3 at a conserved DETD1113/G sequence and its carboxy-terminal inhibitory domain is removed, resulting in deregulated and constitutive kinase activity. The caspase-3-mediated cleavage and activation of ROCK I induces phosphorylation of MLC and membrane blebbing, one of the first events in the execution phase of apoptosis.
UniProt Q13464
Research Area Cancer, Cell Cycle, Cytoskeleton, Microfilaments, Apoptosis/Necrosis, Signaling
Application Notes Western blot: 1 - 2 ug/ml. Recommended Positive Control: camptothecin treated or anti-Fas treated Jurkat (10 ng/ml, 2 hr). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS containing 0.2 % gelatin and 0.05 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.
Expiry Date 12 months
Supplier Images
anti-rho-Associated, Coiled-Coil Containing Protein Kinase 1 (ROCK1) (AA 1114-1126), (cleaved) antibody Detection of cleaved ROCK-1 in apoptotic Jurkat cell lysates (10 ug/lane). Lane 1. Ju...
General Sebbagh, Renvoize, Hamelin et al.: "Caspase-3-mediated cleavage of ROCK I induces MLC phosphorylation and apoptotic membrane blebbing." in: Nature cell biology, Vol. 3, Issue 4, pp. 346-52, 2001 (PubMed).

Amano, Ito, Kimura et al.: "Phosphorylation and activation of myosin by Rho-associated kinase (Rho-kinase)." in: The Journal of biological chemistry, Vol. 271, Issue 34, pp. 20246-9, 1996 (PubMed).

Validation Images
Catalog No. ABIN121298
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