Syntaxin antibody

Antigen: Syntaxin

Clonality: Monoclonal

Host: Mouse

Reactivity: Fish, Mammalian, Xenopus laevis

Application: Western Blotting (WB)

Catalog no.: ABIN121302

Additional Information

Alternative name:

Gene ID: 6804

UniProt: Q16623

Immunogen: Synaptic vesicle-containing fractions that were immunoprecipitated from human brainhomogenates using anti-human synaptophysin monoclonal antibodies (1)

Cross-Reactivity: Fish, Mammalian, Xenopus laevis

Isotype: IgG1

Description: Syntaxin is a 35 kDa type II integral membrane protein localized to the plasma membraneat nerve terminals (2). Syntaxin was identified through its association with synaptotagminand N-type calcium channel, a subtype of calcium channel on the plasma membraneimportant for neurotransmitter release. Homologs of syntaxin were identified in variouseukaryotes ranging from yeast to human (1, 2). Evidences for syntaxin's involvement invesicular transport and neurotransmission include the requirement of its yeast homologs,SSO 1 and 2, in targeting and fusion of Golgi-derived vesicles with the plasma membrane(3). Syntaxin is also the target of clostridial neurotoxin C1 that block neurotransmitterrelease (4). The formation of 20S complex involves the interaction of VAMP with syntaxinthrough an ATP-dependent reaction modulated by synaptotagmin, SNAP-25, alpha-SNAP,and NSF (5).

Application Details

Application Notes: Western blot analysis: 0.1 ug/ml. Recommended Positive Control: human or mouse brain. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 1.0 mg/ml

Purification: Purified

Buffer: PBS, 0.1 mM PMSF and 50 % glycerol

Storage: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoidrepeated freezing and thawing. Shelf life: one year from despatch.

Restrictions: For Research Use only

Images

Western blot analysis of syntaxin in (A) human brain and (B) mouse brain whole cell lysate using ABIN121302 at 0.1 ug/ml.

Publications

Honer, Hu, Davies: "Human synaptic proteins with a heterogeneous distribution in cerebellum and visual cortex." in: Brain research, Vol. 609, Issue 1-2, pp. 9-20, 1993 (PubMed).

Blasi, Chapman, Yamasaki et al.: "Botulinum neurotoxin C1 blocks neurotransmitter release by means of cleaving HPC-1/syntaxin." in: The EMBO journal, Vol. 12, Issue 12, pp. 4821-8, 1993 (PubMed).

Soellner, Bennett, Whiteheart et al.: "A protein assembly-disassembly pathway in vitro that may correspond to sequential steps of synaptic vesicle docking, activation, and fusion." in: Cell, Vol. 75, Issue 3, pp. 409-18, 1993 (PubMed).

Aalto, Ronne, Keraenen: "Yeast syntaxins Sso1p and Sso2p belong to a family of related membrane proteins that function in vesicular transport." in: The EMBO journal, Vol. 12, Issue 11, pp. 4095-104, 1993 (PubMed).

Calakos, Scheller: "Synaptic vesicle biogenesis, docking, and fusion: a molecular description." in: Physiological reviews, Vol. 76, Issue 1, pp. 1-29, 1996 (PubMed).

Honer, Falkai, Young et al.: "Cingulate cortex synaptic terminal proteins and neural cell adhesion molecule in schizophrenia." in: Neuroscience, Vol. 78, Issue 1, pp. 99-110, 1997 (PubMed).