TIN2 (TINF2) (AA 44-58) antibody

Details for Product No. ABIN121307
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Antigen
Epitope
AA 44-58
(5), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(31), (1), (1)
Host
Mouse
(22), (6), (3)
Clonality (Clone)
Monoclonal ()
Application
Western Blotting (WB)
(28), (15), (3), (1), (1), (1)
Pubmed 3 references available
Quantity 0.1 mg
Shipping to United States (Change)
Availability Discontinued
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Catalog No. ABIN121307
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Immunogen Synthetic peptide corresponding to amino acids 44-58 of human Tin2
Clone 59B388
Isotype IgG
Purification Protein G chromatography
Alternative Name TINF2 / TIN2
Background TRF1 is a negative regulator of telomere length maintenance (3). Recently two proteins, tankyrase and Tin2, which bind to mammalian TRF1, have been identified. Tankyrase is a protein with homology to ankyrins as well as to the catalytic domain of poly (adenosine diphosphate-ribose) polymerase (PARP) (3). Tankyrase localizes to telomeres by binding to the telomeric repeat binding factor 1 (TRF1) through its ankyrin repeats (2,3). Tankyrase exhibits PARP activity functioning as acceptors for adenosine diphosphate (ADP)-ribosylation. Since ADP-ribosylation of TRF1 diminishes its ability to bind to telomeric DNA, this suggests that telomere function in human cells is regulated by poly (ADP)-ribosylation. Both the cell cycle and TRF1 may regulate the subcellular localization of tankyrase. The cDNA coding for TIN2 protein was isolated as interacting partner of TRF1 from a yeast two-hybrid cDNA library screening (1). Tin2 localizes to telomeres and is essential for proper regulation of telomere length. Tin2, like TRF1, is widely and constitutively expressed, suggesting that these proteins act together to counterbalance telomere elongation by telomerase. However, endogenous Tin2, like endogenous TRF1, is not highly expressed, and therefore may be difficult to visualize by immunostaining in certain cell systems.
UniProt Q9BSI4
Application Notes Western blot analysis: 1.0 - 3.0 ug/ml. Recommended Positive Control : Daudi. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS, 0.2 % gelatin, 0.05 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.
Expiry Date 12 months
Supplier Images
anti-TIN2 (TINF2) (AA 44-58) antibody Western blot analysis for Tin2 in 293 cells: 10 micrograms per lane 293 cell lysate was resolved by SDS-PAGE and the blot was probed with 2 ug/ml of ABIN121307.
General Smith, Giriat, Schmitt et al.: "Tankyrase, a poly(ADP-ribose) polymerase at human telomeres." in: Science (New York, N.Y.), Vol. 282, Issue 5393, pp. 1484-7, 1998 (PubMed).

Smith, de Lange: "Cell cycle dependent localization of the telomeric PARP, tankyrase, to nuclear pore complexes and centrosomes." in: Journal of cell science, Vol. 112 ( Pt 21), pp. 3649-56, 1999 (PubMed).

Shin, Lee, Kim et al.: "Neuropsychotoxicity of abused drugs: potential of dextromethorphan and novel neuroprotective analogs of dextromethorphan with improved safety profiles in terms of abuse and neuroprotective effects." in: Journal of pharmacological sciences, Vol. 106, Issue 1, pp. 22-7, 2008 (PubMed).

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