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Toll-Like Receptor 2 (TLR2) (Extracellular Domain) antibody

Details for Product No. ABIN121309
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Extracellular Domain
(20), (14), (9), (5), (5), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
(273), (118), (17), (17), (13), (13), (12), (3), (1), (1), (1)
(228), (72), (34), (5), (4), (3)
Clonality (Clone)
Monoclonal ()
(40), (39), (26), (6), (5), (4), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(194), (123), (89), (71), (41), (38), (30), (29), (24), (24), (20), (10), (3), (1), (1), (1)
Pubmed 5 references available
Quantity 0.2 mL
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Catalog No. ABIN121309
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Immunogen Extracellular domain of human TLR2
Clone 1030A5-138
Isotype IgG1
Characteristics Synonyms: Toll-like receptor 2
Purification Ascites
Alternative Name CD282 / TLR2
Background The Toll-like receptor (TLR) family in mammal comprises a family of transmembraneproteins characterized by multiple copies of leucine rich repeats in the extracellulardomain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts inDrosophila, TLRs signal through adaptor molecules (1). The TLR family is a phylogeneticallyconserved mediator of innate immunity that is essential for microbial recognition (2). Tenhuman homologs of TLRs (TLR1-10) have been described (3). TLR2 is differentiallyexpressed in human cells. TLR2 is expressed in tonsils, lymph nodes, and appendices,activated B-cells in germinal centers. CD14+ monocytes expressed the highest level of TLR2followed by CD15+ granulocytes, and CD19+ B-cells, CD3+ T-cells, and CD56+ NK cells didnot express TLR2. The expression of TLR2 on different cell types are regulated by differentimmune response modifiers. For example, LPS, GM-CSF, IL-1, and IL-10 up regulates TLR2whereas IL-4, IFN-gamma, and TNF down regulate TLR2 expression in monocytes (4).
Gene ID 7097
UniProt O60603
Research Area Signaling, Immunology, Inflammation
Application Notes Western blot: 1/250-1/1000. Flow (Intracellular): 2.5-5 µl/10e6 cells. Flow (Cell Surface): 2.5-5 µl/10e6 cells. Immunohistochemistry on Paraffin Sections: 1/100. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Buffer PBS containing 0.05% BSA and 0.05% Sodium Azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Expiry Date 12 months
Supplier Images
anti-Toll-Like Receptor 2 (TLR2) (Extracellular Domain) antibody Western blot analysis of TLR2 in cell lysate from Ramos using ABIN121309 at 1:500 dilution. Fifteen micrograms of Ramos cell lysate was used
anti-Toll-Like Receptor 2 (TLR2) (Extracellular Domain) antibody (2) Flow analysis of TLR2 in 106 ThP1 cells using 5 ul of ABIN121309. The shaded histogram represents ThP1 cells with no anti-TLR2 antibody present, green represents isotype control, purple represents anti-TLR2 antibody.
anti-Toll-Like Receptor 2 (TLR2) (Extracellular Domain) antibody (3) IHC analysis of TLR2 in paraffin-embedded human spleen tissue using ABIN121309 (left) and isotype control (right).
General Medzhitov, Janeway: "Innate immunity: the virtues of a nonclonal system of recognition." in: Cell, Vol. 91, Issue 3, pp. 295-8, 1997 (PubMed).

Muzio, Natoli, Saccani et al.: "The human toll signaling pathway: divergence of nuclear factor kappaB and JNK/SAPK activation upstream of tumor necrosis factor receptor-associated factor 6 (TRAF6)." in: The Journal of experimental medicine, Vol. 187, Issue 12, pp. 2097-101, 1998 (PubMed).

Chuang, Ulevitch: "Cloning and characterization of a sub-family of human toll-like receptors: hTLR7, hTLR8 and hTLR9." in: European cytokine network, Vol. 11, Issue 3, pp. 372-8, 2000 (PubMed).

Flo, Halaas, Torp et al.: "Differential expression of Toll-like receptor 2 in human cells." in: Journal of leukocyte biology, Vol. 69, Issue 3, pp. 474-81, 2001 (PubMed).

Chuang, Ulevitch: "Identification of hTLR10: a novel human Toll-like receptor preferentially expressed in immune cells." in: Biochimica et biophysica acta, Vol. 1518, Issue 1-2, pp. 157-61, 2001 (PubMed).

Validation Images
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