Toll-Like Receptor 2 (TLR2) antibody

Antigen: Toll-Like Receptor 2 (TLR2)

Synonyms: TIL4, CD282, Ly105, TLR2, TLR2A, chTLR2

Clonality: Monoclonal (1030A5-138)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN121309

Additional Information

Alternative name: CD282 / TLR2

Gene ID: 7097

UniProt: O60603

Immunogen: Extracellular domain of human TLR2

Cross-Reactivity: Human

Isotype: IgG1

Clone: 1030A5-138

Description: The Toll-like receptor (TLR) family in mammal comprises a family of transmembraneproteins characterized by multiple copies of leucine rich repeats in the extracellulardomain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts inDrosophila, TLRs signal through adaptor molecules (1). The TLR family is a phylogeneticallyconserved mediator of innate immunity that is essential for microbial recognition (2). Tenhuman homologs of TLRs (TLR1-10) have been described (3). TLR2 is differentiallyexpressed in human cells. TLR2 is expressed in tonsils, lymph nodes, and appendices,activated B-cells in germinal centers. CD14+ monocytes expressed the highest level of TLR2followed by CD15+ granulocytes, and CD19+ B-cells, CD3+ T-cells, and CD56+ NK cells didnot express TLR2. The expression of TLR2 on different cell types are regulated by differentimmune response modifiers. For example, LPS, GM-CSF, IL-1, and IL-10 up regulates TLR2whereas IL-4, IFN-gamma, and TNF down regulate TLR2 expression in monocytes (4).

Characteristics: Synonyms: Toll-like receptor 2

Application Details

Application Notes: Western blot: 1/250-1/1000. Flow (Intracellular): 2.5-5 µl/10e6 cells. Flow (Cell Surface): 2.5-5 µl/10e6 cells. Immunohistochemistry on Paraffin Sections: 1/100. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Purification: Ascites

Buffer: PBS containing 0.05% BSA and 0.05% Sodium Azide.

Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Research Area: Signaling, Immunology, Inflammation

Restrictions: For Research Use only

Images

Western blot analysis of TLR2 in cell lysate from Ramos using ABIN121309 at 1:500 dilution. Fifteen micrograms of Ramos cell lysate was used

Flow analysis of TLR2 in 106 ThP1 cells using 5 ul of ABIN121309. The shaded histogram represents ThP1 cells with no anti-TLR2 antibody present, green represents isotype control, purple represents anti-TLR2 antibody.

IHC analysis of TLR2 in paraffin-embedded human spleen tissue using ABIN121309 (left) and isotype control (right).

Publications

Medzhitov, Janeway: "Innate immunity: the virtues of a nonclonal system of recognition." in: Cell, Vol. 91, Issue 3, pp. 295-8, 1997 (PubMed).

Muzio, Natoli, Saccani et al.: "The human toll signaling pathway: divergence of nuclear factor kappaB and JNK/SAPK activation upstream of tumor necrosis factor receptor-associated factor 6 (TRAF6)." in: The Journal of experimental medicine, Vol. 187, Issue 12, pp. 2097-101, 1998 (PubMed).

Chuang, Ulevitch: "Cloning and characterization of a sub-family of human toll-like receptors: hTLR7, hTLR8 and hTLR9." in: European cytokine network, Vol. 11, Issue 3, pp. 372-8, 2000 (PubMed).

Flo, Halaas, Torp et al.: "Differential expression of Toll-like receptor 2 in human cells." in: Journal of leukocyte biology, Vol. 69, Issue 3, pp. 474-81, 2001 (PubMed).

Chuang, Ulevitch: "Identification of hTLR10: a novel human Toll-like receptor preferentially expressed in immune cells." in: Biochimica et biophysica acta, Vol. 1518, Issue 1-2, pp. 157-61, 2001 (PubMed).