TNF Receptor-Associated Factor 2 (TRAF2) antibody

Antigen: TNF Receptor-Associated Factor 2 (TRAF2)

Synonyms: TRAP, TRAP3, MGC:45012, LAP1, CAP-1, CRAF1, CD40bp, RNF85, MGC:3310, AI325259, amn, T-BAM, AI528849, AI851288, 2310003F17Rik, C630032O20Rik, MGC83471, TANK, TRAF2, LOC716907, LOC100218184, Traf2

Clonality: Monoclonal (33A1293)

Host: Mouse

Reactivity: Human

Application: Western Blotting (WB)

Catalog no.: ABIN121316

Additional Information

Alternative name: TRAF2 / TRAP3

UniProt: Q12933

Immunogen: Fusion protein corresponding to amino acids 205 to 222 of human TRAF2

Cross-Reactivity: Human

Isotype: IgG1

Clone: 33A1293

Description: Tumor necrosis factor (TNF) induced signaling is mediated through association of TNF receptor (TNFR) with adaptor proteins, such as TNF receptor associated proteins (TRAFs). TRAFs form a family of cytoplasmic adapter proteins that mediate signal transduction from many members of the TNF-receptor superfamily (e.g. RANK, CD30, CD40, etc.) and the interleukin-1 receptor. The carboxy-terminal region of TRAFs is required for self-association and interaction with receptor cytoplasmic domains following ligand-induced oligomerization. Recent molecular cloning studies have lead to identification of six TRAFs (TRAF1-TRAF6) (1-4). TRAF2 is a 502-amino acid protein. Mutagenic studies suggest that the N-terminal RING finger and two adjacent zinc fingers of TRAF2 are required for NF-kB activation, where as interaction with TNFR is mediated through C-terminus domain (5,6). Distinct domains in the N- and -C termini are also required for association with TRAF1 and protein kinase receptor interacting protein (RIP). TRAF2 is involved in cellular resistant to TNF-induced apoptosis. TRAF-2 deficient mice appeared normal at birth, however, they die prematurely, probably due to atrophy of thymus, spleen, muscle mass and lack of TRAF-2's cytoprotective role.

Application Details

Application Notes: Western blot analysis: 2.0 ug/ml. Recommended positive control: HeLa (a ~57 kDa band is observed). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.5 mg/ml

Purification: Purified

Buffer: PBS containing 0.2 % gelatin and 0.05 % sodium azide

Storage: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.

Restrictions: For Research Use only

Images

Western blot analysis of 30 ug of HeLa cell lysate with ABIN121316 at 2ug/ml.

Publications

Takeuchi: "[Purification and characterization of alkaline phosphatase obtained from bovine periodontal ligament]" in: Kanagawa shigaku. The Journal of the Kanagawa Odontological Society, Vol. 24, Issue 4, pp. 702-15, 1992 (PubMed).

Cheng, Cleary, Ye et al.: "Involvement of CRAF1, a relative of TRAF, in CD40 signaling." in: Science (New York, N.Y.), Vol. 267, Issue 5203, pp. 1494-8, 1995 (PubMed).

Rothe, Wong, Henzel et al.: "A novel family of putative signal transducers associated with the cytoplasmic domain of the 75 kDa tumor necrosis factor receptor." in: Cell, Vol. 78, Issue 4, pp. 681-92, 1994 (PubMed).

Cao, Xiong, Takeuchi et al.: "TRAF6 is a signal transducer for interleukin-1." in: Nature, Vol. 383, Issue 6599, pp. 443-6, 1996 (PubMed).

Park, Burkitt, Villa et al.: "Structural basis for self-association and receptor recognition of human TRAF2." in: Nature, Vol. 398, Issue 6727, pp. 533-8, 1999 (PubMed).