TNF Receptor-Associated Factor 5 (TRAF5) antibody

Antigen: TNF Receptor-Associated Factor 5 (TRAF5)

Synonyms: RNF84, MGC:39780

Clonality: Monoclonal (55A219)

Host: Mouse

Reactivity: Human, Mouse (Murine)

Application: Western Blotting (WB)

Catalog no.: ABIN121317

Additional Information

Alternative name: TRAF5 / RNF84

UniProt: O00463

Immunogen: Fusion protein corresponding to amino acids 77 to 186 of human TRAF5

Cross-Reactivity: Human, Mouse (Murine)

Isotype: IgG1

Clone: 55A219

Description: Tumor necrosis factor (TNF) induced signaling is mediated through association of TNF receptor (TNFR) with adaptor proteins, such as TNF receptor associated proteins (TRAFs). TRAFs form a family of cytoplasmic adapter proteins that mediate signal transduction from many members of the TNF-receptor superfamily (e.g. RANK, CD30, CD40, etc.) and the interleukin-1 receptor. The carboxy-terminal region of TRAFs is required for self-association and interaction with receptor cytoplasmic domains following ligand-induced oligomerization. Recent molecular cloning studies have lead to identification of six TRAFs (TRAF1-TRAF6) (1-4). TRAF5 is a 558-amino acid protein. TRAF5 is implicated in NF-kB and c-Jun NH(2)-terminal kinase/stress-activated protein kinase activation by members of the TNF receptor superfamily, including CD27, CD30, CD40, and lymphotoxin-beta receptor . Targeted disruption of TRAF5 gene causes defects in CD40- CD27 mediated lymhocyte activation (5).

Application Details

Application Notes: Western blot: 2.0 ug/ml. Recommended positive control: HeLa. In HeLa, a ~64 kDa band is observed. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.5 mg/ml

Purification: Purified

Buffer: PBS containing 0.2 % gelatin, 0.05 % sodium azide

Storage: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.

Restrictions: For Research Use only

Images

Western blot analysis of TRAF5 using ABIN121317 at 2 ug/ml dilution against 10 ug of HeLa cell lysate.

Publications

Cheng, Cleary, Ye et al.: "Involvement of CRAF1, a relative of TRAF, in CD40 signaling." in: Science (New York, N.Y.), Vol. 267, Issue 5203, pp. 1494-8, 1995 (PubMed).

Rothe, Wong, Henzel et al.: "A novel family of putative signal transducers associated with the cytoplasmic domain of the 75 kDa tumor necrosis factor receptor." in: Cell, Vol. 78, Issue 4, pp. 681-92, 1994 (PubMed).

Cao, Xiong, Takeuchi et al.: "TRAF6 is a signal transducer for interleukin-1." in: Nature, Vol. 383, Issue 6599, pp. 443-6, 1996 (PubMed).

Nakano, Sakon, Koseki et al.: "Targeted disruption of Traf5 gene causes defects in CD40- and CD27-mediated lymphocyte activation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, Issue 17, pp. 9803-8, 1999 (PubMed).