Add to Basket
|+1 404 474 4654|
|+1 888 205 9894 (TF)|
TNF Receptor-Associated Factor 5 (TRAF5) antibody
Human, Mouse (Murine)
Alternatives Western Blotting (WB)
|4 references available|
|Quantity||0.1 mg (0.5 mg/ml)|
|Price||352.00 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 6 to 8 Business Days|
|Alternative name||TRAF5 / RNF84|
|Immunogen||Fusion protein corresponding to amino acids 77 to 186 of human TRAF5|
|Cross-Reactivity||Human, Mouse (Murine)|
|Description||Tumor necrosis factor (TNF) induced signaling is mediated through association of TNF receptor (TNFR) with adaptor proteins, such as TNF receptor associated proteins (TRAFs). TRAFs form a family of cytoplasmic adapter proteins that mediate signal transduction from many members of the TNF-receptor superfamily (e.g. RANK, CD30, CD40, etc.) and the interleukin-1 receptor. The carboxy-terminal region of TRAFs is required for self-association and interaction with receptor cytoplasmic domains following ligand-induced oligomerization. Recent molecular cloning studies have lead to identification of six TRAFs (TRAF1-TRAF6) (1-4). TRAF5 is a 558-amino acid protein. TRAF5 is implicated in NF-kB and c-Jun NH(2)-terminal kinase/stress-activated protein kinase activation by members of the TNF receptor superfamily, including CD27, CD30, CD40, and lymphotoxin-beta receptor . Targeted disruption of TRAF5 gene causes defects in CD40- CD27 mediated lymhocyte activation (5).|
|Application Notes||Western blot: 2.0 ug/ml. Recommended positive control: HeLa. In HeLa, a ~64 kDa band is observed. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.|
|Buffer||PBS containing 0.2 % gelatin, 0.05 % sodium azide|
|Storage||Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.|
|Restrictions||For Research Use only|
|Western blot analysis of TRAF5 using ABIN121317 at 2 ug/ml dilution against 10 ug of HeLa cell lysate.|
Cheng, Cleary, Ye et al.: "Involvement of CRAF1, a relative of TRAF, in CD40 signaling." in: Science (New York, N.Y.), Vol. 267, Issue 5203, pp. 1494-8, 1995 (PubMed).
Rothe, Wong, Henzel et al.: "A novel family of putative signal transducers associated with the cytoplasmic domain of the 75 kDa tumor necrosis factor receptor." in: Cell, Vol. 78, Issue 4, pp. 681-92, 1994 (PubMed).
Cao, Xiong, Takeuchi et al.: "TRAF6 is a signal transducer for interleukin-1." in: Nature, Vol. 383, Issue 6599, pp. 443-6, 1996 (PubMed).
Nakano, Sakon, Koseki et al.: "Targeted disruption of Traf5 gene causes defects in CD40- and CD27-mediated lymphocyte activation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, Issue 17, pp. 9803-8, 1999 (PubMed).
|Hosts||Rabbit (10), Mouse (3)|
|Reactivities||Human (10), Mouse (Murine) (6), Rat (Rattus) (4), Gerbil (1)|
|Applications||Western Blotting (WB) (12), ELISA (6), Immunohistochemistry (IHC) (5), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (4), Immunoprecipitation (IP) (2), Immunohistochemistry (Fixed) (IHC (fx)) (1), Immunohistochemistry (Frozen Sections) (IHC (fro)) (1)|
|Epitopes||AA 77-186 (3), AA 1-20 (2)|