Tumor Necrosis Factor (Ligand) Superfamily, Member 11 (TNFSF11) antibody

Antigen: Tumor Necrosis Factor (Ligand) Superfamily, Member 11 (TNFSF11)

Synonyms: ODF, OPGL, sOdf, CD254, OPTB2, RANKL, TRANCE, hRANKL2, TNFSF11

Clonality: Monoclonal (12A668)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Catalog no.: ABIN121319

Additional Information

Alternative name: CD254 / RANKL

UniProt: O14788

Immunogen: Bacterially expressed fusion protein containing amino acid residues 1-317 of mouse TRANCE

Cross-Reactivity: Human, Mouse (Murine)

Isotype: IgG1

Clone: 12A668

Description: TRANCE/OPGL/RANKL/ODF is a recently identified member of tumor necrosis factor family. TRANCE (TNF-related activation-induced cytokine receptor) or RANKL {receptor activator of NF-kB (RANK) ligand} has been implicated in interactions between T cells and dendritic cells (1-2). RANK ligand (RANKL/TRANCE) binds to RANK on dendritic cells, upregulates the expression of anti-apoptotic protein BcL-XL suggesting a role in dendritic cell survival (2). TRANCE/RANKL is also important in T- and B-cell maturation (3). As OPGL/ODF (Osteoprotegerin Ligand/Osteoclast differentiation factor), the same protein can both activate mature osteoclasts and mediate osteoclastogenesis (4). OPGL/TRANCE deficient mice show severe osteoporesis and complete absence of osteoclasts as a result of lack of osteogenesis (3).

Specificity: This antibody is specififc for Trance/RANKL/OPGL/ODF.

Application Details

Application Notes: Western blot analysis: 1.0 - 2.0 ug/ml. Immunohistochemistry on paraffin embedded sections. Recommended Positive Control: activated T cells or RAW. This mAb does not work for flow cytometry. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.5 mg/ml

Purification: Purified

Buffer: PBS containing 0.05 % BSA and 0.05 % sodium azide

Storage: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.

Restrictions: For Research Use only

Images

Western blot analysis of TRANCE/RANKL in transfected cells. A single protein band of approximate mol. wt. of 35 kDa was detected.

Publications

Anderson, Maraskovsky, Billingsley et al.: "A homologue of the TNF receptor and its ligand enhance T-cell growth and dendritic-cell function." in: Nature, Vol. 390, Issue 6656, pp. 175-9, 1997 (PubMed).

Wong, Josien, Lee et al.: "TRANCE (tumor necrosis factor [TNF]-related activation-induced cytokine), a new TNF family member predominantly expressed in T cells, is a dendritic cell-specific survival factor." in: The Journal of experimental medicine, Vol. 186, Issue 12, pp. 2075-80, 1998 (PubMed).

Kong, Yoshida, Sarosi et al.: "OPGL is a key regulator of osteoclastogenesis, lymphocyte development and lymph-node organogenesis." in: Nature, Vol. 397, Issue 6717, pp. 315-23, 1999 (PubMed).