Tumor Necrosis Factor (Ligand) Superfamily, Member 11 (TNFSF11) antibody

Antigen: Tumor Necrosis Factor (Ligand) Superfamily, Member 11 (TNFSF11)

Synonyms: ODF, OPGL, sOdf, CD254, OPTB2, RANKL, TRANCE, hRANKL2, TNFSF11

Clonality: Monoclonal (12A380)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC)

Catalog no.: ABIN121320

Additional Information

Alternative name: CD254 / RANKL

UniProt: O14788

Immunogen: A bacterially expressed fusion protein containing amino acid residues 1-317 of mouse TRANCE

Cross-Reactivity: Human, Mouse (Murine)

Isotype: IgG1

Clone: 12A380

Description: TRANCE is a member of tumor necrosis factor family. TRANCE or RANKL has been implicated in interactions between T cells and dendritic cells (ref. 4-5). RANK ligand (RANKL/TRANCE) binds to RANK on dendritic cells, upregulates the expression of anti-apoptotic protein BcL-XL suggesting a role in dendritic cell survival (ref. 4). TRANCE/RANKL is also important in T- and B-cell maturation (ref. 3). As OPGL/ODF (Osteoprotegerin Ligand/Osteoclast differentiation factor), the same protein can both activate mature osteoclasts and mediate osteoclastogenesis (ref. 1,2). OPGL/TRANCE deficient mice show severe osteoporesis and complete absence of osteoclasts as a result of lack of osteogenesis (ref. 3). Alternate Names: RANKL (receptor activator of NF-kB (RANK) ligand), OPGL, ODF.

Specificity: The antibody recognizes TRANCE / RANKL.

Application Details

Application Notes: Western blotting (4-6 ug/ml). Immunohistochemistry on frozen sections (ref. 6). Flow cytometry (Cell Surface, ref. 6). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.5 mg/ml

Purification: Purified

Buffer: PBS containing 0.05% BSA and 0.05% sodium azide

Storage: Store the antibody at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.

Restrictions: For Research Use only

Images

Western blot analysis of TRANCE in transfected cells using SM7110 at 5 ug/ml.

Publications

Anderson, Maraskovsky, Billingsley et al.: "A homologue of the TNF receptor and its ligand enhance T-cell growth and dendritic-cell function." in: Nature, Vol. 390, Issue 6656, pp. 175-9, 1997 (PubMed).

Wong, Josien, Lee et al.: "TRANCE (tumor necrosis factor [TNF]-related activation-induced cytokine), a new TNF family member predominantly expressed in T cells, is a dendritic cell-specific survival factor." in: The Journal of experimental medicine, Vol. 186, Issue 12, pp. 2075-80, 1998 (PubMed).

Lacey, Timms, Tan et al.: "Osteoprotegerin ligand is a cytokine that regulates osteoclast differentiation and activation." in: Cell, Vol. 93, Issue 2, pp. 165-76, 1998 (PubMed).

Kong, Yoshida, Sarosi et al.: "OPGL is a key regulator of osteoclastogenesis, lymphocyte development and lymph-node organogenesis." in: Nature, Vol. 397, Issue 6717, pp. 315-23, 1999 (PubMed).

Hsu, Lacey, Dunstan et al.: "Tumor necrosis factor receptor family member RANK mediates osteoclast differentiation and activation induced by osteoprotegerin ligand." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, Issue 7, pp. 3540-5, 1999 (PubMed).