Telomeric Repeat Binding Factor 2 (TERF2) antibody

Details for Product No. ABIN121321
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Deer, Human, Mouse (Murine), Rat (Rattus)
(57), (13), (6), (3), (3), (2), (1)
(40), (12), (11)
Clonality (Clone)
Monoclonal ()
(2), (2), (2), (2), (2), (2), (1), (1), (1)
Immunohistochemistry (Frozen Sections) (IHC (fro)), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB), Flow Cytometry (FACS)
(57), (20), (16), (11), (10), (10), (9), (4), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1)
Pubmed 2 references available
Quantity 0.1 mg
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Catalog No. ABIN121321
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Immunogen Baculovirus expressed whole length TRF2 protein was used for immunizing and splenocytes used to generate the hybridoma clone
Clone 4A794-15
Isotype IgG1
Purification Protein G chromatography
Alternative Name TERF2
Background TRF2 is a ubiquitously expressed protein that is implicated in the control of telomere length (1). TRF2, like TRF1 contains a Myb-related DNA binding motif. It binds to duplex TTAGGG repeats and is localized to all human telomeres in metaphase chromosomes (1). TRF2 is thought to protect chromosome ends by maintaining the correct structure at telomere termini (2). The use of mutant forms of TRF2 has implicated a role for TRF2 in the prevention of senescence in primary human cells (2). Recently, it has been shown that inhibition of TRF2 resulted in apoptosis in a subset of mammalian cell types (3).
UniProt Q15554
Research Area DNA/RNA, Cell Cycle, Enzymes
Application Notes Western blot: 2.0 ug/ml. Immunoprecipitation: 2 ug/1X10e6 cells. ELISA: 2 ug/ml. Immunocytochemistry/Immunofluorescence. Immunohistochemistry on frozen sections: 5 ug/ml. Recommended positive control: HL60 (The antibody recognizes distinct doublet bands of approximate size 66 kDa corresponding to the molecular size of huTRF2 by western blot analysis against lysates from most human cell lines). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS containing 0.05 % BSA and 0.05 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.
Expiry Date 12 months
Supplier Images
anti-Telomeric Repeat Binding Factor 2 (TERF2) antibody Immunoprecipitation (IP)/ Western Blot (WB) Analysis of TRF2 in HL60 cells: (Lane 1) IP with IMG-124 (mouse anti-TRF2). (Lane 2) IP with control mouse IgG. (Lane 3) IP with IMG-148 (goat anti-TRF2). (Lane 4) IP with pre-immune goat Ig. (Lanes 1-4) WB with ABIN121321. TRF2 is detected as an ~ 66 kD protein.
General Bradshaw, Stavropoulos, Meyn: "Human telomeric protein TRF2 associates with genomic double-strand breaks as an early response to DNA damage." in: Nature genetics, Vol. 37, Issue 2, pp. 193-7, 2005 (PubMed).

Broccoli, Smogorzewska, Chong et al.: "Human telomeres contain two distinct Myb-related proteins, TRF1 and TRF2." in: Nature genetics, Vol. 17, Issue 2, pp. 231-5, 1997 (PubMed).

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