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|+1 888 205 9894 (TF)|
Toll-Like Receptor 6 (TLR6) antibody
|Synonyms||CD286, TLR6, TLR1, TLR1LA|
Alternatives Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
|4 references available|
|Quantity||0.1 mg (0.5 mg/ml)|
|Price||330.00 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 6 to 8 Business Days|
|Alternative name||CD286 / TLR6|
|Immunogen||A synthetic peptide corresponding to amino acids 408-424 of human TRL6|
|Description||The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules (1). The TRL family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition (2). TLRs characterized so far activate the MyD88/interleukin-1 receptor-associated kinase (IRAK) signaling pathway. Ten human homologs of TLRs (TLR1-10) have been described. The amino acid sequence of human TLR6 is most similar to hTLR1 with 69% identity at the amino acid level. Human TLR6 consists of a predicted 807 amino acids with a molecular weight of approximately 92 kDa (3). Human and mouse TLR6 share an amino acid identity of 73%. TLR6 activates both NF-kB and c-Jun N- terminal Kinase (JNK). The amino acids in the cytoplasmic domain of IL-1 receptor, which are critical for NF-kB activation, are conserved in TLR6. It has also been shown that TLR2-mediated response to a phenol-soluble factor from staphylococcus epidermidis is enhanced by TLR6 (4). TLR6 is predominantly expressed in spleen, thymus, ovary, and lung (3). Alternate Names: TLR-6, Toll-like Receptor 6, FocusOn004.|
|Application Notes||Flow cytometry (Intracellular): 3-5 ug / 10e6 cells. Ramos cells can be used as a positive control. Flow Cytometry (Cell surface): see Wong et al. (2007) for details. Immunohistochemistry on Paraffin Sections: 5 ug/ml. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.|
|Buffer||PBS containing 0.05% Sodium Azide as preservative and 0.05% BSA as stabilizer.|
|Storage||Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.|
|Research Area||Signaling, Immunology, Inflammation|
|Restrictions||For Research Use only|
|Intracellular FACS analysis of TLR6 (shaded peak) and mouse IgG1 isotype control (open peak) in 106 Ramos cells using 3 ug of ABIN121322|
Medzhitov, Janeway: "Innate immunity: the virtues of a nonclonal system of recognition." in: Cell, Vol. 91, Issue 3, pp. 295-8, 1997 (PubMed).
Muzio, Natoli, Saccani et al.: "The human toll signaling pathway: divergence of nuclear factor kappaB and JNK/SAPK activation upstream of tumor necrosis factor receptor-associated factor 6 (TRAF6)." in: The Journal of experimental medicine, Vol. 187, Issue 12, pp. 2097-101, 1998 (PubMed).
Takeuchi, Kawai, Sanjo et al.: "TLR6: A novel member of an expanding toll-like receptor family." in: Gene, Vol. 231, Issue 1-2, pp. 59-65, 1999 (PubMed).
Hajjar, OMahony, Ozinsky et al.: "Cutting edge: functional interactions between toll-like receptor (TLR) 2 and TLR1 or TLR6 in response to phenol-soluble modulin." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 166, Issue 1, pp. 15-9, 2001 (PubMed).