Toll-Like Receptor 6 (TLR6) antibody

Antigen: Toll-Like Receptor 6 (TLR6)

Synonyms: CD286, TLR6, TLR1, TLR1LA

Clonality: Monoclonal (86B1153)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN121322

Additional Information

Alternative name: CD286 / TLR6

UniProt: Q9Y2C9

Immunogen: A synthetic peptide corresponding to amino acids 408-424 of human TRL6

Cross-Reactivity: Human

Isotype: IgG1

Clone: 86B1153

Description: The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules (1). The TRL family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition (2). TLRs characterized so far activate the MyD88/interleukin-1 receptor-associated kinase (IRAK) signaling pathway. Ten human homologs of TLRs (TLR1-10) have been described. The amino acid sequence of human TLR6 is most similar to hTLR1 with 69% identity at the amino acid level. Human TLR6 consists of a predicted 807 amino acids with a molecular weight of approximately 92 kDa (3). Human and mouse TLR6 share an amino acid identity of 73%. TLR6 activates both NF-kB and c-Jun N- terminal Kinase (JNK). The amino acids in the cytoplasmic domain of IL-1 receptor, which are critical for NF-kB activation, are conserved in TLR6. It has also been shown that TLR2-mediated response to a phenol-soluble factor from staphylococcus epidermidis is enhanced by TLR6 (4). TLR6 is predominantly expressed in spleen, thymus, ovary, and lung (3). Alternate Names: TLR-6, Toll-like Receptor 6, FocusOn004.

Application Details

Application Notes: Flow cytometry (Intracellular): 3-5 ug / 10e6 cells. Ramos cells can be used as a positive control. Flow Cytometry (Cell surface): see Wong et al. (2007) for details. Immunohistochemistry on Paraffin Sections: 5 ug/ml. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.5 mg/ml

Purification: Purified

Buffer: PBS containing 0.05% Sodium Azide as preservative and 0.05% BSA as stabilizer.

Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.

Research Area: Signaling, Immunology, Inflammation

Restrictions: For Research Use only

Images

Intracellular FACS analysis of TLR6 (shaded peak) and mouse IgG1 isotype control (open peak) in 106 Ramos cells using 3 ug of ABIN121322

Publications

Medzhitov, Janeway: "Innate immunity: the virtues of a nonclonal system of recognition." in: Cell, Vol. 91, Issue 3, pp. 295-8, 1997 (PubMed).

Muzio, Natoli, Saccani et al.: "The human toll signaling pathway: divergence of nuclear factor kappaB and JNK/SAPK activation upstream of tumor necrosis factor receptor-associated factor 6 (TRAF6)." in: The Journal of experimental medicine, Vol. 187, Issue 12, pp. 2097-101, 1998 (PubMed).

Takeuchi, Kawai, Sanjo et al.: "TLR6: A novel member of an expanding toll-like receptor family." in: Gene, Vol. 231, Issue 1-2, pp. 59-65, 1999 (PubMed).

Hajjar, OMahony, Ozinsky et al.: "Cutting edge: functional interactions between toll-like receptor (TLR) 2 and TLR1 or TLR6 in response to phenol-soluble modulin." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 166, Issue 1, pp. 15-9, 2001 (PubMed).