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Details for Product No. ABIN121325

Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA) (Cleavage Site) antibody

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Antigen
Synonyms IKBA, MAD-3, NFKBI, AI462015, Nfkbi, RL/IF-1, ECI-6/IKBA
Epitope
»Alternatives Cleavage Site
Reactivity
»Alternatives Human
Host
»Alternatives Mouse
Clonality (Clone) Monoclonal ()
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives Immunoprecipitation (IP), Western Blotting (WB)
Pubmed 8 references available
Catalog no. ABIN121325
Quantity 0.1 mg
Price
489.50 $   Plus shipping costs $45.00
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Availability Discontinued
Immunogen Synthetic peptide corresponding to the caspase-3 mediated cleavage site of IkBa
Clone 5D1623
Isotype IgG1
Purification Protein G Chromatography
Alternative Name NFKBIA / IKBA
Background NF-kB is silenced in the cytoplasm by an inhibitory protein, IkB (1). Synthesis of IkBa is autoregulated (2). IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g (3-6). External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Six members of IkB family members have been identified (1). Recent studies have shown that IkBa can be cleaved by caspases during apoptosis to produce an amino-terminal truncated IkBa (DN-IkBa) (7,8). The DN-IkBa is resistant to degradation in response to inducers of NF-kB and act as dominant inhibitory molecule that suppresses NF-kB activity during apoptosis.
UniProt P25963
Application Notes Western blot: 1.0 ug/ml. Immunoprecipitation: 1.0 - 2.0 ug/ml. Recommended positive control: anti-Fas treated Jurkat (30 min). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS containing 0.02 % sodium azide
Preservative Sodium azide
Storage 4 °C/-20 °C
Storage Comment Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.
Expiry Date 12 months
Verma, Stevenson, Schwarz et al.: "Rel/NF-kappa B/I kappa B family: intimate tales of association and dissociation." in: Genes & development, Vol. 9, Issue 22, pp. 2723-35, 1995 (PubMed).

Régnier, Song, Gao et al.: "Identification and characterization of an IkappaB kinase." in: Cell, Vol. 90, Issue 2, pp. 373-83, 1997 (PubMed).

DiDonato, Hayakawa, Rothwarf et al.: "A cytokine-responsive IkappaB kinase that activates the transcription factor NF-kappaB." in: Nature, Vol. 388, Issue 6642, pp. 548-54, 1997 (PubMed).

Verma, Stevenson: "IkappaB kinase: beginning, not the end." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 22, pp. 11758-60, 1997 (PubMed).

Mercurio, Zhu, Murray et al.: "IKK-1 and IKK-2: cytokine-activated IkappaB kinases essential for NF-kappaB activation." in: Science (New York, N.Y.), Vol. 278, Issue 5339, pp. 860-6, 1997 (PubMed).

Barkett, Xue, Horvitz et al.: "Phosphorylation of IkappaB-alpha inhibits its cleavage by caspase CPP32 in vitro." in: The Journal of biological chemistry, Vol. 272, Issue 47, pp. 29419-22, 1997 (PubMed).

Yamaoka, Courtois, Bessia et al.: "Complementation cloning of NEMO, a component of the IkappaB kinase complex essential for NF-kappaB activation." in: Cell, Vol. 93, Issue 7, pp. 1231-40, 1998 (PubMed).

Reuther, Baldwin: "Apoptosis promotes a caspase-induced amino-terminal truncation of IkappaBalpha that functions as a stable inhibitor of NF-kappaB." in: The Journal of biological chemistry, Vol. 274, Issue 29, pp. 20664-70, 1999 (PubMed).

Alternatives for antigen "Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA)", type "Antibodies"
Hosts (230), (46), (6)
Reactivities (270), (161), (136), (43), (40), (15), (14), (1)
Applications (200), (103), (76), (59), (50), (40), (36), (13), (11), (6), (5), (3), (3), (2), (2), (1)
Conjugates (11), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4)
Epitopes (47), (43), (33), (20), (19), (14), (6), (5), (5), (4), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
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