Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA) (Cleavage Site) antibody

Details for Product No. ABIN121325
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Antigen
Epitope
Cleavage Site
(55), (46), (37), (30), (26), (23), (22), (15), (15), (13), (13), (12), (7), (5), (5), (4), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(378), (187), (160), (6), (4), (4), (2), (2), (1)
Host
Mouse
(336), (48), (7)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(16), (12), (10), (7), (6), (6), (4), (4), (4), (4), (4), (4), (4), (4)
Application
Immunoprecipitation (IP), Western Blotting (WB)
(278), (146), (131), (62), (55), (51), (40), (20), (17), (15), (9), (5), (5), (4), (1), (1), (1)
Pubmed 8 references available
Quantity 0.1 mg
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Immunogen Synthetic peptide corresponding to the caspase-3 mediated cleavage site of IkBa
Clone 5D1623
Isotype IgG1
Purification Protein G Chromatography
Alternative Name NFKBIA / IKBA (NFKBIA Antibody Abstract)
Background NF-kB is silenced in the cytoplasm by an inhibitory protein, IkB (1). Synthesis of IkBa is autoregulated (2). IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g (3-6). External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Six members of IkB family members have been identified (1). Recent studies have shown that IkBa can be cleaved by caspases during apoptosis to produce an amino-terminal truncated IkBa (DN-IkBa) (7,8). The DN-IkBa is resistant to degradation in response to inducers of NF-kB and act as dominant inhibitory molecule that suppresses NF-kB activity during apoptosis.
UniProt P25963
Application Notes Western blot: 1.0 ug/ml. Immunoprecipitation: 1.0 - 2.0 ug/ml. Recommended positive control: anti-Fas treated Jurkat (30 min). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS containing 0.02 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.
Expiry Date 12 months
Supplier Images
anti-Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA) (Cleavage Site) antibody Detection of cleaved IkBalpha in Jurkat cell lysates. Lane A. untreated cell lysate. ...
General Reuther, Baldwin: "Apoptosis promotes a caspase-induced amino-terminal truncation of IkappaBalpha that functions as a stable inhibitor of NF-kappaB." in: The Journal of biological chemistry, Vol. 274, Issue 29, pp. 20664-70, 1999 (PubMed).

Yamaoka, Courtois, Bessia et al.: "Complementation cloning of NEMO, a component of the IkappaB kinase complex essential for NF-kappaB activation." in: Cell, Vol. 93, Issue 7, pp. 1231-40, 1998 (PubMed).

Barkett, Xue, Horvitz et al.: "Phosphorylation of IkappaB-alpha inhibits its cleavage by caspase CPP32 in vitro." in: The Journal of biological chemistry, Vol. 272, Issue 47, pp. 29419-22, 1997 (PubMed).

Mercurio, Zhu, Murray et al.: "IKK-1 and IKK-2: cytokine-activated IkappaB kinases essential for NF-kappaB activation." in: Science (New York, N.Y.), Vol. 278, Issue 5339, pp. 860-6, 1997 (PubMed).

Verma, Stevenson: "IkappaB kinase: beginning, not the end." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 22, pp. 11758-60, 1997 (PubMed).

DiDonato, Hayakawa, Rothwarf et al.: "A cytokine-responsive IkappaB kinase that activates the transcription factor NF-kappaB." in: Nature, Vol. 388, Issue 6642, pp. 548-54, 1997 (PubMed).

Régnier, Song, Gao et al.: "Identification and characterization of an IkappaB kinase." in: Cell, Vol. 90, Issue 2, pp. 373-83, 1997 (PubMed).

Verma, Stevenson, Schwarz et al.: "Rel/NF-kappa B/I kappa B family: intimate tales of association and dissociation." in: Genes & development, Vol. 9, Issue 22, pp. 2723-35, 1995 (PubMed).

Validation Images
Catalog No. ABIN121325
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