Tumor Protein P73 (TP73) antibody

Antigen: Tumor Protein P73 (TP73)

Synonyms: TP73, P73, Trp73, tp73, p73, Tp73, TAp73, zp73theta

Clonality: Monoclonal (5B429)

Host: Mouse

Reactivity: Human, Mouse (Murine)

Conjugate: Un-conjugated

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)

Catalog no.: ABIN121331

Additional Information

UniProt: O15350

Immunogen: Full-length human p73

Cross-Reactivity: Human, Mouse (Murine)

Isotype: IgG

Clone: 5B429

Description: P73 was identified as a long-lost cousin of tumor suppressor protein, p53 (4). p73 has high homology with p53 as well as with p63, a gene implicated in the maintenance of epithelial stem cells. Significant homology between p53, p63, and p73 (approximately 63% amino acid identity in the DNA-binding domain suggest that they may have overlapping functions in the regulation of gene expression (3). The targeted disruption of p73 gene leads to defects hippocampal dysgenesis, hydrocephalus, chronic inflammation and infections (2). Recently, spilicing variant mRNAs of p73 has been identified in MCF-7, a breast carcinoma cell line. These mRNAs code for variant p73 proteins bearing distinct carboxy-terminal structures suggesting that the carboxy- terminal region of p73 may be important for the functions of this protein (1). Tumor BioMarker: Vella et al (2003) found p73 to be upregulated in a significant fraction of anaplastic thyroid cancers, whereas it was not detectable in normal thyroid epithelial cells nor in papillary or follicular thyroid cancer.

Application Details

Application Notes: Western blot analysis: 2 ug/ml. Immunoprecipitation: Please see Sayan et al (2005) for details. Immunofluorescence/Immunocytochemistry: Please see Vella et al (2003) and Sayan et al (2005) for details. IHC (frozen): See Dominguez et al (2006) for details. Recommended positive control: p73 transfected cells. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Purification: Purified

Buffer: PBS containing 0.05 % BSA and 0.05 % sodium azide

Storage: Store the antibody (in aliquots) at -20 °C. Can be shipped at 2 - 8 °C. Avoid repeated freezing and thawing. Centrifuge vial before opening. Shelf Life: One year from despatch.

Restrictions: For Research Use only

Images

Western blot analysis of p73 in A) transfected cell lysate and B) HeLa cell lysate using ABIN121331 at 2

Publications

Kaghad, Bonnet, Yang et al.: "Monoallelically expressed gene related to p53 at 1p36, a region frequently deleted in neuroblastoma and other human cancers." in: Cell, Vol. 90, Issue 4, pp. 809-19, 1997 (PubMed).

Yang, Walker, Bronson et al.: "p73-deficient mice have neurological, pheromonal and inflammatory defects but lack spontaneous tumours." in: Nature, Vol. 404, Issue 6773, pp. 99-103, 2000 (PubMed).

Levrero, De Laurenzi, Costanzo et al.: "The p53/p63/p73 family of transcription factors: overlapping and distinct functions." in: Journal of cell science, Vol. 113 ( Pt 10), pp. 1661-70, 2000 (PubMed).

Minagawa, Honda, Miyazaki et al.: "Comparative proteomic and transcriptomic profiling of the human hepatocellular carcinoma." in: Biochemical and biophysical research communications, Vol. 366, Issue 1, pp. 186-92, 2007 (PubMed).