Caspase 1, Apoptosis-Related Cysteine Peptidase (Interleukin 1, Beta, Convertase) (CASP1) antibody

Antigen: Caspase 1, Apoptosis-Related Cysteine Peptidase (Interleukin 1, Beta, Convertase) (CASP1)

Synonyms: ICE, P45, IL1BC, Il1bc, Ice, DCP-1, DCP1, l(2)01862, l(2)02132, DmelCG5370, CG5370, CASP1, LOC100033888

Clonality: Monoclonal (14F468)

Host: Mouse

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN121334

Additional Information

Alternative name: Caspase-1

UniProt: P29466

Immunogen: Synthetic peptide corresponding to amino acids 371-390 of human caspase-1 AA Sequence:RKVRFSFEQPDGRAQMPTTE

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus)

Isotype: IgG1

Clone: 14F468

Description: Caspases are a family of cysteine proteases that are key mediators of programmed cell death or apoptosis (1). The precursor form of all caspases is composed of a prodomain, and large and small catalytic subunits. The active forms of caspases are generated by several stimuli including ligand-receptor interactions, growth factor deprivation and inhibitors of cellular functions. All known caspases require cleavage adjacent to aspartates to liberate one large and one small subunit, which associate into tetramer to form the active enzyme. Caspase-1/ICE (IL-1b converting enzyme) is similar to the cell death gene CED-3 of Caenorhabditilis elegans and regulates multiple proinflammatory cytokines, including interleukin-1b and interferon- gamma-inducing factor.

Application Details

Application Notes: Western blot (0.5-2 ug/ml). Immunohistochemistry on paraffin embedded sections. Recommended Positive Control: HeLa or NIH 3T3 (a 45 kDa band is observed). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.5 mg/ml

Purification: Purified

Buffer: PBS containing 0.2 % gelatin and 0.05 % sodium azide

Storage: Store the antibody (in aliquots) at -20 °C. Can be shipped at 2 - 8 °C. Avoid repeated freezing and thawing. Centrifuge vial before opening. Shelf Life: One year from despatch.

Research Area: Apoptosis/Necrosis

Restrictions: For Research Use only

Images

Western blot analysis of Caspase-1 using ABIN121334 at 0.5 ug/ml on (A) HeLa cells and (B) at 2 ug/ml NIH3T3 cells.

Formalin-fixed, paraffin-embedded sections of human colon cancer stained for caspase-1 expression (ABIN121334) using a DAB chromogen and Hematoxylin counterstain. 2 sections, from different human patients were stained. Differential staining was observed. Only rare staining in A is observed. In contrast, abundant staining is observed in B

Publications

Cerretti, Kozlosky, Mosley et al.: "Molecular cloning of the interleukin-1 beta converting enzyme." in: Science (New York, N.Y.), Vol. 256, Issue 5053, pp. 97-100, 1992 (PubMed).

Li, Allen, Banerjee et al.: "Mice deficient in IL-1 beta-converting enzyme are defective in production of mature IL-1 beta and resistant to endotoxic shock." in: Cell, Vol. 80, Issue 3, pp. 401-11, 1995 (PubMed).

Yuan, Shaham, Ledoux et al.: "The C. elegans cell death gene ced-3 encodes a protein similar to mammalian interleukin-1 beta-converting enzyme." in: Cell, Vol. 75, Issue 4, pp. 641-52, 1993 (PubMed).

Tatsuta, Shiraishi, Mountz: "The prodomain of caspase-1 enhances Fas-mediated apoptosis through facilitation of caspase-8 activation." in: The Journal of biological chemistry, Vol. 275, Issue 19, pp. 14248-54, 2000 (PubMed).