Caspase 1 (CASP1) (AA 371-390) antibody

Details for Product No. ABIN121334
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Antigen
Epitope
AA 371-390
(33), (15), (8), (7), (7), (6), (6), (5), (4), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine), Rat (Rattus)
(176), (50), (42), (5), (4), (1), (1), (1), (1), (1)
Host
Mouse
(184), (6), (5), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(12), (5), (5), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(165), (111), (77), (57), (15), (15), (14), (13), (12), (10), (5), (3), (2), (1)
Pubmed 4 references available
Quantity 0.1 mg
Shipping to United States (Change)
Availability Discontinued
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Catalog No. ABIN121334
500.50 $
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Immunogen Synthetic peptide corresponding to amino acids 371-390 of human caspase-1 AA Sequence:RKVRFSFEQPDGRAQMPTTE
Clone 14F468
Isotype IgG1
Purification Protein G chromatography
Alternative Name Caspase-1
Background Caspases are a family of cysteine proteases that are key mediators of programmed cell death or apoptosis (1). The precursor form of all caspases is composed of a prodomain, and large and small catalytic subunits. The active forms of caspases are generated by several stimuli including ligand-receptor interactions, growth factor deprivation and inhibitors of cellular functions. All known caspases require cleavage adjacent to aspartates to liberate one large and one small subunit, which associate into tetramer to form the active enzyme. Caspase-1/ICE (IL-1b converting enzyme) is similar to the cell death gene CED-3 of Caenorhabditilis elegans and regulates multiple proinflammatory cytokines, including interleukin-1b and interferon- gamma-inducing factor.
UniProt P29466
Research Area Apoptosis/Necrosis
Application Notes Western blot (0.5-2 ug/ml). Immunohistochemistry on paraffin embedded sections. Recommended Positive Control: HeLa or NIH 3T3 (a 45 kDa band is observed). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS containing 0.2 % gelatin and 0.05 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store the antibody (in aliquots) at -20 °C. Can be shipped at 2 - 8 °C. Avoid repeated freezing and thawing. Centrifuge vial before opening. Shelf Life: One year from despatch.
Expiry Date 12 months
Supplier Images
anti-Caspase 1 (CASP1) (AA 371-390) antibody Western blot analysis of Caspase-1 using ABIN121334 at 0.5 ug/ml on (A) HeLa cells and (B) at 2 ug/ml NIH3T3 cells.
anti-Caspase 1 (CASP1) (AA 371-390) antibody (2) Formalin-fixed, paraffin-embedded sections of human colon cancer stained for caspase-1 expression (ABIN121334) using a DAB chromogen and Hematoxylin counterstain. 2 sections, from different human patients were stained. Differential staining was observed. Only rare staining in A is observed. In contrast, abundant staining is observed in B
General Cerretti, Kozlosky, Mosley et al.: "Molecular cloning of the interleukin-1 beta converting enzyme." in: Science (New York, N.Y.), Vol. 256, Issue 5053, pp. 97-100, 1992 (PubMed).

Li, Allen, Banerjee et al.: "Mice deficient in IL-1 beta-converting enzyme are defective in production of mature IL-1 beta and resistant to endotoxic shock." in: Cell, Vol. 80, Issue 3, pp. 401-11, 1995 (PubMed).

Yuan, Shaham, Ledoux et al.: "The C. elegans cell death gene ced-3 encodes a protein similar to mammalian interleukin-1 beta-converting enzyme." in: Cell, Vol. 75, Issue 4, pp. 641-52, 1993 (PubMed).

Tatsuta, Shiraishi, Mountz: "The prodomain of caspase-1 enhances Fas-mediated apoptosis through facilitation of caspase-8 activation." in: The Journal of biological chemistry, Vol. 275, Issue 19, pp. 14248-54, 2000 (PubMed).

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