Toll-Like Receptor 4 (TLR4) antibody

Antigen: Toll-Like Receptor 4 (TLR4)

Synonyms: TOLL, CD284, hToll, ARMD10, Lps, Ly87, Ran/M1, Rasl2-8

Clonality: Monoclonal (76B357-1)

Host: Mouse

Reactivity: Cow (Bovine), Chimpanzee, Horse (Equine), Human, Mouse (Murine), Pig (Porcine), Rat (Rattus), Sheep (Ovine)

Conjugate: Un-conjugated

Application: Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN121335

Additional Information

Alternative name: CD284 / TLR4

UniProt: O00206

Immunogen: A portion of amino acids 100-200 of human TLR4

Cross-Reactivity: Cow (Bovine), Chimpanzee, Horse (Equine), Human, Mouse (Murine), Pig (Porcine), Rat (Rattus), Sheep (Ovine)

Isotype: IgG2b

Clone: 76B357-1

Description: The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules (1). The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition (2). Ten human homologs of TLRs (TLR1-10) have been described (3). Among this family of receptors, TLR2 and TLR4 have been most studied. These studies have suggested that TLR2 and TLR4 may serve as potential main mediators of LPS signaling (4,5). The TLR4 cDNA codes for a protein consisting of 799 amino acids with approximate molecular weight of 88 kDa (6). Alternate Names: TLR-4, hToll, Toll-like Receptor 4, FocusOn004.

Application Details

Application Notes: Flow cytometry (cell surface): 0.5-2 ug/10e6 cells. Recommended positive control: ThP1, Ramos. Western blot: 1-3 ug/ml. Recommended positive control: Intestine lysate. Immunohistochemistry on paraffin sections: 5 ug/ml. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.5 mg/ml

Purification: Purified

Buffer: PBS containing 0.05% BSA and 0.05% sodium azide

Storage: Stable at 2-8°C for up to one month or at -20°C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch. General.

Research Area: Signaling, Immunology, Inflammation

Restrictions: For Research Use only

Images

Western blot analysis of TLR4 using ABIN121335 at 2 ug/ml on (A) human intestine and (B)mouseintestine.

Flow analysis of TLR4 in 10^6 ThP1 cells using 5 ug of ABIN121335. Shaded histogram represents ThP1 cells without anti-TLR4 antibody, green represents isotype control, purple represents anti-TLR4 antibody.

Publications

Medzhitov, Janeway: "Innate immunity: the virtues of a nonclonal system of recognition." in: Cell, Vol. 91, Issue 3, pp. 295-8, 1997 (PubMed).

Muzio, Natoli, Saccani et al.: "The human toll signaling pathway: divergence of nuclear factor kappaB and JNK/SAPK activation upstream of tumor necrosis factor receptor-associated factor 6 (TRAF6)." in: The Journal of experimental medicine, Vol. 187, Issue 12, pp. 2097-101, 1998 (PubMed).

Takeuchi, Hoshino, Kawai et al.: "Differential roles of TLR2 and TLR4 in recognition of gram-negative and gram-positive bacterial cell wall components." in: Immunity, Vol. 11, Issue 4, pp. 443-51, 1999 (PubMed).

Poltorak, Ricciardi-Castagnoli, Citterio et al.: "Physical contact between lipopolysaccharide and toll-like receptor 4 revealed by genetic complementation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, Issue 5, pp. 2163-7, 2000 (PubMed).

Chuang, Ulevitch: "Identification of hTLR10: a novel human Toll-like receptor preferentially expressed in immune cells." in: Biochimica et biophysica acta, Vol. 1518, Issue 1-2, pp. 157-61, 2001 (PubMed).