APIP (APIP) antibody

Details for Product No. ABIN121336
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

 
Antigen
Synonyms APIP2, CGI29, MMRP19, dJ179L10.2, hAPIP, CGI-29, Mmrp19, RGD1564562
Reactivity
Human
(40), (15), (15), (11), (11), (11), (11)
Host
Mouse
(34), (7)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(30), (12), (9), (8), (5)
Pubmed 4 references available
Catalog no. ABIN121336
Quantity 0.1 mg
Price
456.50 $   Plus shipping costs $45.00
Shipping to United States (Change)
Availability Discontinued
Add to Basket

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen Full-length His-tagged recombinant APIP protein
Clone 19F461
Isotype IgG2b
Purification Protein G chromatography
Alternative Name APIP
Background The mammalian homologues of the key cell death gene CED-4 in C. elegans has been identified recently from human and mouse and designated Apaf1 (for apoptosis protease-activating factor 1) (1,2). Apaf1 binds to cytochrome c (Apaf2) and caspase-9 (Apaf3), which leads to caspase-9 activation. Activated caspase-9 in turn cleaves and activates caspase-3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis3. Recently, Cho et al (4) have identified a new Apaf-1 Interactiong Protein (APIP) also known as CG129 and MMRP19, as a negative regulator of ischemic injury. APIP competes with Caspase-9 binding site of Apaf1. APIP is predicted to code for a 204 amino acid. An isoform of APIP, APIP2 encodes a 242 amino acid protein, which is an alternative splicing variant differing in its N- terminus from APIP. APIP transcript is ubiquitously expressed in most adult tissue with high expression in skeletal muscle, heart, and kidney. Alternate Names: CG129 / MMRP19.
UniProt Q96GX9
Research Area Apoptosis/Necrosis
Application Notes Western blot analysis: 1.0 - 2.0 ug/ml. Recommended Positive Control : HeLa or 293. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS containing 0.2 % gelatin and 0.05 % sodium azide
Preservative Sodium azide
Storage -20 °C
Storage Comment Store the antibody (in aliquots) at -20 °C. Can be shipped at 2 - 8 °C. Avoid repeated freezing and thawing. Centrifuge vial before opening. Shelf Life: One year from despatch.
Expiry Date 12 months
Supplier Images
anti-APIP (APIP) antibody Western blot analysis of APIP2 in A) recombinant protein, B) HeLa, and C) 293 whole cell lysate using ABIN121336 at 2 ug/ml.
General Zou, Henzel, Liu et al.: "Apaf-1, a human protein homologous to C. elegans CED-4, participates in cytochrome c-dependent activation of caspase-3." in: Cell, Vol. 90, Issue 3, pp. 405-13, 1997 (PubMed).

Li, Nijhawan, Budihardjo et al.: "Cytochrome c and dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade." in: Cell, Vol. 91, Issue 4, pp. 479-89, 1997 (PubMed).

Cecconi, Alvarez-Bolado, Meyer et al.: "Apaf1 (CED-4 homolog) regulates programmed cell death in mammalian development." in: Cell, Vol. 94, Issue 6, pp. 727-37, 1998 (PubMed).

Nguyen, Lee, Cho et al.: "Crystallization and Preliminary X-Ray Studies of TON_0559, a Putative Member of the Haloacid Dehalogenase (HAD) Superfamily from Thermococcus onnurineus NA1." in: Protein and peptide letters, Vol. 15, Issue 2, pp. 235-7, 2008 (PubMed).

Hosts (34), (7)
Reactivities (40), (15), (15), (11), (11), (11), (11)
Applications (30), (12), (9), (8), (5)
Conjugates (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (1), (1), (1), (1), (1), (1)
back to top