SARS N (0) antibody

Antigen: SARS N (0)

Clonality: Monoclonal (Ncap11)

Host: Mouse

Reactivity: Human

Application: Immunofluorescence (IF), Western Blotting (WB)

Catalog no.: ABIN121338

Additional Information

Characteristics: Synonyms: SARS-CoV N, Severe acute respiratory syndrome coronavirus Nucleoprotein, Nucleocapsidprotein, Protein N, NC, 9a

Alternative name: SARS N

Gene ID: 1489678

Swiss-Prot: P59595

Immunogen: Full-length recombinant SARS Nucleocapsid Protein. Remarks: Genbank accession no. NP_828858

Isotype: IgG  (Matching secondary antibodies)

Clone: Ncap11

Description: It has recently been shown that SARS is caused by a human coronavirus. Humancoronaviruses are the major cause of upper respiratory tract illness in humans, such as thecommon cold. Coronaviruses are positive-stranded RNA viruses, featuring the largest viralRNA genomes known to date (27-31 kb). The first step in coronavirus infection is binding ofthe viral spike protein, a 139-kDa protein, to certain receptors on host cells. The spikeprotein is the main surface antigen of the coronavirus. The most prominent protein in theculture supernatants infected with SARS virus is a 46 kDa nucleocapsid protein. Thissuggests that the nucleocapsid protein is a major immunogen that may be useful for earlydiagnostics.

Specificity: This antibody is specific for SARS-Nucleocapsid protein. Species: Human. Others not tested.

Application Details

Application Notes: Western blot analysis: 1/500-1/1000 (Detects a band of ~46 kDa). Immunofluorescence/Immunocytochemistry: 1/500. Recommended Positive Control: Transfected Mouse melanoma cell lysate. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.5 mg/ml

Buffer: PBS containing 0.2% Gelatin as stabilizer and 0.05% Sodium Azide aspreservative.

Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: One year from despatch.

Restrictions: For Research Use only

Images

Western blot analysis of SARS Nucleocapsid in (A) transfected mouse melanoma cell lysate and (B) untransfected cell lysate using ABIN121338 at 1:1000.

Transfected HeLa cells were stained with ABIN121338 at 1:500 dilution.

Publications

Publications: Rota, Oberste, Monroe et al.: "Characterization of a novel coronavirus associated with severe acute respiratory syndrome." in: Science (New York, N.Y.), Vol. 300, Issue 5624, pp. 1394-9, 2003 (PubMed).

Krokhin, Li, Andonov et al.: "Mass spectrometric characterization of proteins from the SARS virus: a preliminary report." in: Molecular & cellular proteomics : MCP, Vol. 2, Issue 5, pp. 346-56, 2003 (PubMed).