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DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA) (N-Term) antibody

Antigen

DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA)
Synonyms DFF1, ICAD, DFF-45, DFF35, Dff45, ICAD-L, ICAD-S, A330085O09Rik, DFFA, MGC143066, DKFZp469D0625
Binding Site
Alternatives

N-Term
Clonality Polyclonal
Host
Alternatives

Rabbit
Reactivity
Alternatives

Mouse (Murine)
Application
Alternatives Western Blotting (WB)
2 references available
Catalog no. ABIN121553
Quantity 0.1 mg  (1.0 mg/ml)  (Variants)
Price 330.00 $   Plus shipping costs $45.00
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Additional Information
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Alternative name DFFA / ICAD
UniProt O54786
Immunogen Synthetic peptide corresponding to amino acids 2-21 of mouse ICAD
Cross-Reactivity Mouse (Murine)
Description ICAD (Inhibitor of Caspase-Activated Dnase) is the murine homologue of human DFF-45. ICAD is cleaved by Caspase-3, releasing CAD which eventually causes degradation of DNA in the nucleus. Alternate Names: Inhibitor of CAD, DFF-45, DFF1, DNA fragmentation factor subunit alpha, DNA fragmentation factor 45 kDa subunit.
Specificity This antibody reacts to ICAD at C-term.

Application Details
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Application Notes Western blot: 1/1000, this antibody recognises a 45kD band in Western blots. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Concentration 1.0 mg/ml
Purification Purified
Buffer PBS containing 0.02% Sodium Azide
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.
Research Area Apoptosis/Necrosis
Restrictions For Research Use only

Publications
Application Details Alternatives Variants back to top

Wyllie: "Apoptosis. An endonuclease at last." in: Nature, Vol. 391, Issue 6662, pp. 20-1, 1998 (PubMed).

Enari, Sakahira, Yokoyama et al.: "A caspase-activated DNase that degrades DNA during apoptosis, and its inhibitor ICAD." in: Nature, Vol. 391, Issue 6662, pp. 43-50, 1998 (PubMed).

Alternatives
Application Details Publications Variants back to top

Alternatives for antigen "DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA)", type "Antibodies"
Hosts Rabbit (84), Mouse (12), Goat (1)
Reactivities Human (65), Mouse (Murine) (37), Rat (Rattus) (7), Cat (Feline) (1), Chicken (1), Cow (Bovine) (1), Dog (Canine) (1)
Applications Western Blotting (WB) (91), ELISA (30), Immunohistochemistry (IHC) (16), Immunoprecipitation (IP) (12), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (11), Immunofluorescence (IF) (8), Immunohistochemistry (Fixed) (IHC (fx)) (3), Immunocytochemistry (ICC) (2), Dot Blot (Dot) (1), Immunohistochemistry (Frozen Sections) (IHC (fro)) (1)
Epitopes N-Term (20), C-Term (15), AA 2-21 (7), AA 312-331 (5), AA 313-331 (3), Transcript Variant 1 (3), AA 1-331 (2), Internal Region (2), N-Term,AA 2-21 (2), AA 31-2 (1), AA 312-331,C-Term (1), Asp224 (1), Cleaved-Asp224 (1)