DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA) (AA 312-331), (C-Term) antibody

Details for Product No. ABIN121557
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Antigen
Synonyms DFFA, DFF-45, DFF1, ICAD, A330085O09Rik, DFF35, Dff45, ICAD-L, ICAD-S
Epitope
AA 312-331, C-Term
(42), (35), (14), (9), (8), (8), (7), (5), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Mouse (Murine)
(144), (74), (24), (2)
Host
Rabbit
(174), (26), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(172), (74), (31), (31), (20), (18), (18), (10), (7), (4), (2), (1)
Pubmed 2 references available
Quantity 0.1 mg
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Catalog No. ABIN121557
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Immunogen Synthetic peptide corresponding to amino acids 312-331 of mouse ICAD
Purification Affinity chromatography
Alternative Name DFFA / ICAD
Background ICAD (Inhibitor of Caspase-Activated DNase) is the murine homologue of human DFF-45. ICAD is cleaved by Caspase-3, releasing CAD which eventually causes degradation of DNA in the nucleus. Alternate Names: Inhibitor of CAD, DFF-45, DFF1, DNA fragmentation factor subunit alpha, DNA fragmentation factor 45 kDa subunit.
UniProt O54786
Research Area Apoptosis/Necrosis
Application Notes Western Blot: 1/500 - 1/1000, this antibody recognises a 45kD band in Western blots. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS containing 0.02% Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.
Expiry Date 12 months
General Wyllie: "Apoptosis. An endonuclease at last." in: Nature, Vol. 391, Issue 6662, pp. 20-1, 1998 (PubMed).

Enari, Sakahira, Yokoyama et al.: "A caspase-activated DNase that degrades DNA during apoptosis, and its inhibitor ICAD." in: Nature, Vol. 391, Issue 6662, pp. 43-50, 1998 (PubMed).

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