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DFFA antibody (DNA Fragmentation Factor, 45kDa, alpha Polypeptide) (AA 312-331)

Details for Product anti-DFFA Antibody No. ABIN121557, Supplier: Log in to see
Antigen
  • DFFA
  • DFF-45
  • DFF1
  • ICAD
  • A330085O09Rik
  • DFF35
  • Dff45
  • ICAD-L
  • ICAD-S
Alternatives
anti-Mouse (Murine) DFFA antibody for Immunohistochemistry (Paraffin-embedded Sections)
Epitope
AA 312-331, C-Term
37
28
18
16
13
12
11
9
5
4
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Mouse (Murine)
158
72
31
1
1
Host
Rabbit
183
33
2
Clonality
Polyclonal
Conjugate
This DFFA antibody is un-conjugated
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
178
72
40
24
24
20
11
11
6
4
4
3
2
1
1
1
Supplier
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Immunogen Synthetic peptide corresponding to amino acids 312-331 of mouse ICAD
Specificity This antibody reacts to ICAD.
Purification Affinity chromatography
Alternative Name DFFA / ICAD (DFFA Antibody Abstract)
Background ICAD (Inhibitor of Caspase-Activated DNase) is the murine homologue of human DFF-45. ICAD is cleaved by Caspase-3, releasing CAD which eventually causes degradation of DNA in the nucleus.Synonyms: DFF-45, DFF1, DFF45, DNA fragmentation factor 45 kDa subunit, DNA fragmentation factor subunit alpha, Inhibitor of CAD
UniProt O54786
Research Area Apoptosis/Necrosis
Pathways Apoptosis, Caspase Cascade in Apoptosis
Application Notes Western Blot: 1/500 - 1/1000, this antibody recognises a 45 kD band in Western blots.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 0.5 mg/mL
Buffer PBS containing 0.02 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch.
Expiry Date 12 months
Background publications Enari, Sakahira, Yokoyama et al.: "A caspase-activated DNase that degrades DNA during apoptosis, and its inhibitor ICAD." in: Nature, Vol. 391, Issue 6662, pp. 43-50, 1998 (PubMed).

Wyllie: "Apoptosis. An endonuclease at last." in: Nature, Vol. 391, Issue 6662, pp. 20-1, 1998 (PubMed).